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dc.contributor.author
Anderson, H. Clarke
dc.contributor.author
Reynolds, Paul R.
dc.contributor.author
Tsu, Howard H. T,
dc.contributor.author
Missana, Liliana Raquel
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dc.contributor.author
Masuhara, Kensaky
dc.contributor.author
Moylan, Paul E.
dc.contributor.author
Roach, Helmtrude I.
dc.date.available
2020-03-27T14:45:46Z
dc.date.issued
2002-12
dc.identifier.citation
Anderson, H. Clarke; Reynolds, Paul R.; Tsu, Howard H. T,; Missana, Liliana Raquel; Masuhara, Kensaky; et al.; Selective Synthesis of Bone Morphogenetic proteins 1, -3, -4 and Bone Sialoprotein may be important for osteoinduction by Saos-2 cells; Springer; Journal Of Bone And Mineral Metabolism; 20; 12-2002; 73-82
dc.identifier.issn
0914-8779
dc.identifier.uri
http://hdl.handle.net/11336/101058
dc.description.abstract
An ability to induce new bone formation at a required site would represent a considerable advance in bone repair and tissue engineering. It has been shown that the healing of critical-size bone defects in rats can be augmented by extracts of Saos-2 cells. These human osteosarcoma cells uniquely contain a bone-inducing activity, whereas other human osteosarcoma cells, e.g., U-2 OS cells, cannot replicate the osteoinductive capacity. To understand the necessary components of the Saos-2 bone-inducing activity, this study compared osteoinductive Saos-2 cells with non-osteoinductive U-2 OS cells with respect to the synthesis of bone morphogenetic proteins (BMPs)-1, -2, -3, -4, -5, -6, and -7 and the non-collagenous matrix proteins bone sialoprotein (BSP), osteonectin (ON), osteopontin (OPN), and osteocalcin (OC). The main differences were abundant synthesis of BMP-1/tolloid, BMP-3, -4, and BSP by Saos-2 cells, but absence or reduced synthesis in U-2 OS cells. BMP-2 and -7 were present in low amounts in both cell types, while BMP-5 and -6 were more abundant in U-2 OS cells, suggesting that these BMPs were of lesser importance for the osteoinductivity of Saos-2 cells. However, a relatively high expression of BMP-3 and -4, together with BMP-1/tolloid, may be important for the osteoinductive capacity of Saos-2 cells. The inability of U2-OS cells to induce bone, despite expressing most of the BMPs, may be due to an insufficiency of tolloid, BMP-3 or -4, BSP, and/or other unknown factors. A better understanding of the necessary components of the Saos-2 cell bone-inducing agent may, in future, lead to clinically useful Saos-2 cell products for bone repair and tissue engineering.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
bone induction
dc.subject
Saos 2-cells
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Bone Morphogenetic proteins 1, -3, -4
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Bone Sialoprotein
dc.subject.classification
Odontología, Medicina y Cirugía Oral
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dc.subject.classification
Medicina Clínica
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dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
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dc.title
Selective Synthesis of Bone Morphogenetic proteins 1, -3, -4 and Bone Sialoprotein may be important for osteoinduction by Saos-2 cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-03-25T14:38:55Z
dc.journal.volume
20
dc.journal.pagination
73-82
dc.journal.pais
Japón
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dc.journal.ciudad
Tokio
dc.description.fil
Fil: Anderson, H. Clarke. University of Kansas; Estados Unidos
dc.description.fil
Fil: Reynolds, Paul R.. University of Kansas; Estados Unidos
dc.description.fil
Fil: Tsu, Howard H. T,. University of Kansas; Estados Unidos
dc.description.fil
Fil: Missana, Liliana Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina. University of Kansas; Estados Unidos
dc.description.fil
Fil: Masuhara, Kensaky. University of Kansas; Estados Unidos
dc.description.fil
Fil: Moylan, Paul E.. University of Kansas; Estados Unidos
dc.description.fil
Fil: Roach, Helmtrude I.. University of Southampton; Reino Unido
dc.journal.title
Journal Of Bone And Mineral Metabolism
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