Artículo
Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells
Fecha de publicación:
09/2018
Editorial:
Lifescience Global
Revista:
Journal of Nutritional Therapeutics
ISSN:
1929-5634
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Non-muscle myosin IIA is a cytoplasmic protein that works in concert with F-actin to produce cell movement. The heavy chain of this protein is codified by the MYH9 gene. The presence of motor proteins as myosin or mono and F-actin and their role in transcription has recently been observed. Prep1–the transcription factor of HOXB genes– constitutes a dimer with Pbx1, which induces HOXB gene expression. Prep1 has been found purifying with β-actin and Myh9. HOXB transcription initiates when cells enter in S-phase, during which DNA duplication and transcription occur at the same time. Here, we have shown that Myh9 co-localizes with Prep1 in the nucleus and in the periphery of the nucleolus in S-phase NT2-D1 cells. Furthermore, we have shown that Myh9 purifies with Pbx1 from nuclear extracts of S-phase entering NT2-D1 cells –and not from cytoplasmic extracts. Taking into account these results, we conclude that Myh9 is in the nucleus of the S-phase entering NT2-D1 cells and might have a role in HOXB transcription.
Palabras clave:
Myosin9
,
Hox genes
,
NT2-D1 cells
,
S-phase
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Citación
Naum Ongania, Gabriela; Rivera Pomar, Rolando; Non-Muscle Myosin IIA (Myh9) is in the Nucleus of S-Phase Entering NT2-D1 Cells; Lifescience Global; Journal of Nutritional Therapeutics; 7; 2; 9-2018; 59-66
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