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Artículo

Proteome variation of the rat liver after static cold storage assayed in an ex vivo model

Knecht, Camila Ayelén; Balaban, Cecilia LucíaIcon ; Rodríguez, Joaquín V.; Ceccarelli, Eduardo AugustoIcon ; Guibert, Edgardo ElvioIcon ; Rosano, German LeandroIcon
Fecha de publicación: 12/2018
Editorial: Academic Press Inc Elsevier Science
Revista: Cryobiology
ISSN: 0011-2240
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
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Resumen

Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver proteins associated with injury during cold storage and/or normothermic reperfusion using the isolated perfused rat liver model. Livers from male rats were subjected to either (1) cold storage for 24 h, (2) ex vivo normothermic reperfusion for 90 min or (3) cold storage for 24 h followed by ex vivo normothermic reperfusion for 90 min. Then, the livers were homogenized and proteins were extracted. Protein expression between each experimental group and the control (freshly resected livers) was compared by two-dimensional (2D) gel electrophoresis. Protein identification was carried out by matrix‐assisted laser desorption/ionization time‐of‐flight spectrometry (MALDI‐TOF/TOF) using MASCOT as the search engine. 23 proteins were detected with significantly altered levels of expression among the different treatments, including molecular chaperones, antioxidant enzymes, and proteins involved in energy metabolism. Some of them have been postulated as biomarkers for liver damage while others had been identified in other organs subjected to ischemia and reperfusion injury. The whole data set will be a useful resource for studying deleterious molecular mechanisms that result in diminished liver function during storage and subsequent reperfusion.
Palabras clave: COLD ISCHEMIA , LIVER , PROTEOMICS , REPERFUSION INJURY , TWO-DIMENSIONAL GEL ELECTROPHORESIS
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/100789
URL: https://www.sciencedirect.com/science/article/pii/S0011224018301731
DOI: https://doi.org/10.1016/j.cryobiol.2018.10.003
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Knecht, Camila Ayelén; Balaban, Cecilia Lucía; Rodríguez, Joaquín V.; Ceccarelli, Eduardo Augusto; Guibert, Edgardo Elvio; et al.; Proteome variation of the rat liver after static cold storage assayed in an ex vivo model; Academic Press Inc Elsevier Science; Cryobiology; 85; 12-2018; 47-55
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