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dc.contributor.author
Contreras Esquivel, J.C.
dc.contributor.author
Voget, Claudio Enrique
dc.date.available
2020-03-25T15:02:17Z
dc.date.issued
2004-05
dc.identifier.citation
Contreras Esquivel, J.C.; Voget, Claudio Enrique; Purification and partial characterization of an acidic polygalacturonase from Aspergillus kawachii; Elsevier Science; Journal of Biotechnology; 110; 1; 5-2004; 21-28
dc.identifier.issn
0168-1656
dc.identifier.uri
http://hdl.handle.net/11336/100677
dc.description.abstract
An endo-polygalacturonase, named PGI, was purified to homogeneity from the culture filtrate of Aspergillus kawachii IFO 4033 grown in a glucose-tryptone medium. The molecular mass of PGI was estimated to be 60kDa by SDS-PAGE and 40kDa by gel filtration on Sephacryl S-100. The isoelectric point was 3.55 as determined by isoelectic focusing. PGI exhibited binding properties to ConA-Sepharose suggesting that the protein is glycosylated. The N-terminal amino acid sequence was also determined as S-T-C-T-F-T-D-A-A-T-A-S-E-S-K. The remarkable property of PGI was its high activity in the pH range 2.0-3.0 towards soluble and insoluble substrates, while being inactive at pH 5.0. Enzyme stability at low pHs was markedly enhanced by different compounds, such as proteins, polysaccharides, simple sugars and the substrate pectin. PGI was very efficient to extract pectin from lemmon protopectin and to macerate carrot tissues at pH 2.0. These properties make PGI an interesting biocatalyst for industrial applications under highly acidic conditions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ACIDIC ENZYMES
dc.subject
ASPERGILLUS KAWACHII
dc.subject
ENDO-POLYGALACTURONASE
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PECTIN
dc.subject.classification
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación
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Biotecnología Industrial
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Purification and partial characterization of an acidic polygalacturonase from Aspergillus kawachii
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-03-16T15:01:47Z
dc.journal.volume
110
dc.journal.number
1
dc.journal.pagination
21-28
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Contreras Esquivel, J.C.. Universidad Autonoma de Coahuila; México
dc.description.fil
Fil: Voget, Claudio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina
dc.journal.title
Journal of Biotechnology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0168165604000616
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.jbiotec.2004.01.010
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