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dc.contributor.author
Devine, C.
dc.contributor.author
Brennan, G.P.
dc.contributor.author
Lanusse, Carlos Edmundo
dc.contributor.author
Alvarez, Luis Ignacio
dc.contributor.author
Trudgett, A.
dc.contributor.author
Hoey, E.
dc.contributor.author
Fairweather, I.
dc.date.available
2020-03-19T21:05:56Z
dc.date.issued
2009-02
dc.identifier.citation
Devine, C.; Brennan, G.P.; Lanusse, Carlos Edmundo; Alvarez, Luis Ignacio; Trudgett, A.; et al.; Effect of the metabolic inhibitor, methimazole on the drug susceptibility of a triclabendazole-resistant isolate of Fasciola hepatica; Cambridge University Press; Parasitology; 136; 2; 2-2009; 183-192
dc.identifier.issn
0031-1820
dc.identifier.uri
http://hdl.handle.net/11336/100371
dc.description.abstract
A study has been carried out to investigate whether the action of triclabendazole (TCBZ) is altered in the presence of a metabolic inhibitor. The flavin monooxygenase system (FMO) was inhibited using methimazole (MTZ) to see whether a TCBZ-resistant isolate could be made more sensitive to TCBZ action. The Oberon TCBZ-resistant and Cullompton TCBZ-sensitive isolates were used for these experiments. The FMO system was inhibited by a 2-h pre-incubation in methimazole (100 m). Flukes were then incubated for a further 22 h in NCTC medium containing either MTZ; MTZ+nicotinamide adenine dinucleotide phosphate (NADPH) (1 nm); MTZ+NADPH+TCBZ (15 g/ml); or MTZ+NADPH+triclabendazole sulphoxide (TCBZ.SO) (15 g/ml). Morphological changes resulting from drug treatment and following metabolic inhibition were assessed using scanning electron microscopy. After treatment with either TCBZ or TCBZ.SO alone, there was greater surface disruption to the triclabendazole-susceptible than -resistant isolate. However, co-incubation with MTZ and TCBZ/TCBZ.SO lead to more severe surface changes to the TCBZ-resistant isolate than with each drug on its own; this was not seen for the TCBZ-susceptible Cullompton isolate. Results of this study support the concept of altered drug metabolism in TCBZ-resistant flukes and this process may play a role in the development of drug resistance.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
FASCIOLA HEPATICA
dc.subject
LIVER FLUKE
dc.subject
METHIMAZOLE
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SCANNING ELECTRON MICROSCOPY
dc.subject
TRICLABENDAZOLE RESISTANCE
dc.subject.classification
Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Effect of the metabolic inhibitor, methimazole on the drug susceptibility of a triclabendazole-resistant isolate of Fasciola hepatica
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-11-08T15:12:05Z
dc.journal.volume
136
dc.journal.number
2
dc.journal.pagination
183-192
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Devine, C.. The Queens University of Belfast; Irlanda
dc.description.fil
Fil: Brennan, G.P.. The Queens University of Belfast; Irlanda
dc.description.fil
Fil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Fisiopatología. Laboratorio de Farmacología; Argentina
dc.description.fil
Fil: Trudgett, A.. The Queens University of Belfast; Irlanda
dc.description.fil
Fil: Hoey, E.. The Queens University of Belfast; Irlanda
dc.description.fil
Fil: Fairweather, I.. The Queens University of Belfast; Irlanda
dc.journal.title
Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/parasitology/article/effect-of-the-metabolic-inhibitor-methimazole-on-the-drug-susceptibility-of-a-triclabendazoleresistant-isolate-of-fasciola-hepatica/8F408B63974A7A834ADA46601948EB51
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1017/S0031182008005222
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