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dc.contributor.author
Cerioli, Maria Florencia
dc.contributor.author
Moliva, Melina Vanesa
dc.contributor.author
Cariddi, Laura Noelia
dc.contributor.author
Reinoso, Elina Beatríz
dc.date.available
2020-03-19T18:07:09Z
dc.date.issued
2018-07
dc.identifier.citation
Cerioli, Maria Florencia; Moliva, Melina Vanesa; Cariddi, Laura Noelia; Reinoso, Elina Beatríz; Effect of the essential oil of Minthostachys verticillata (Griseb.) epling and limonene on biofilm production in pathogens causing bovine mastitis; Frontiers Research Foundation; Frontiers in Veterinary Science; 5; JUL; 7-2018; 146-154
dc.identifier.issn
2297-1769
dc.identifier.uri
http://hdl.handle.net/11336/100287
dc.description.abstract
Bovine mastitis causes large annual economic losses around the world. Different microorganisms are associated with the disease. The capacity of pathogens to adhere to bovine mammary epithelial cells is associated with biofilm production which leads to antibiotic resistance. Research is now leading to search alternative control methods and medicinal plants constitute a natural, safe, effective and inexpensive option. Minthostachys verticillata is an autochthonous medicinal plant of Argentina with multiple ethnobotanical properties. In a previous study, we demonstrated that the essential oil (EO) of this species and limonene, one of its compounds, inhibited the growth of mastitis pathogens. The objective of the present work was to determine the inhibitory effect of the essential oil of M. verticillata and limonene, on biofilm formation and on mature biofilm produced by pathogens isolated from bovine mastitis. Time kill assay and bacterial lysis were also determined. Furthermore, RAPD-PCR assays were performed to determine changes in bacterial DNA after EO and limonene exposition. Bacterial isolates were identified as Escherichia coli (EC3 and EC9), Bacillus pumilus (BP5, BP6, and BP7) and Enterococcus faecium (EF1) by rRNA 16S sequencing and MALDI-TOF MS. All the strains were able to form biofilm. Addition of both lactose and sucrose did not affect biofilm production. MIC values for EO were 3.6 mg/ml for E. faecium; 0.9 mg/ml for E. coli (EC3), 14.5 mg/ml for E. coli (EC9), 1.8 mg/ml for B. pumilus (BP7), 3.63 mg/ml for B. pumilus (BP6) and 29.0 mg/ml for B. pumilus (BP7). MIC values for limonene were 6.6 mg/ml for B. pumilus (BP6) and 105 mg/ml for B. pumilus (BP5). These results demonstrated that EO was more effective than limonene, showing also bactericidal action against E. faecium (minimal inhibitory concentration (MBC) = 29.0 mg/ml). This result was corroborated by time of death assay, observing a cell decrease after at 6 h, and then by bacterial lysis assay. Both EO and limonene affected mature biofilm of isolated strains. The results contribute to the study of EO and limonene which may serve as a therapy against bovine mastitis pathogens inhibiting the development of pathogenic bacteria.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Frontiers Research Foundation
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BOVINE MASTITIS
dc.subject
ESSENTIAL OIL
dc.subject
INHIBITORY EFFECT
dc.subject
LIMONENE
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MINTHOSTACHYS VERTICILLATA
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Effect of the essential oil of Minthostachys verticillata (Griseb.) epling and limonene on biofilm production in pathogens causing bovine mastitis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-03-13T18:05:41Z
dc.journal.volume
5
dc.journal.number
JUL
dc.journal.pagination
146-154
dc.journal.pais
Suiza
dc.description.fil
Fil: Cerioli, Maria Florencia. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Moliva, Melina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
dc.description.fil
Fil: Cariddi, Laura Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
dc.description.fil
Fil: Reinoso, Elina Beatríz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Río Cuarto; Argentina
dc.journal.title
Frontiers in Veterinary Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6037896/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2018.00146/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3389/fvets.2018.00146
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