Allele frequencies of 15 STR loci in the population of the City of Quito, Ecuador

Abstract

Whole blood obtained by venipuncture was collected from unre-lated individuals residing in Quito City, Ecuador, South America,using one 1.2-mm micropunch of bloodstains collected on FTAClassic Cards (Whatman FTAÒ3), and the DNA was extractedfollowing the manufacturer’s directions. PCR amplification of the15 STR loci (D3S1358, THO1, D21S11, D18S51, PENTA E,D5S818, D13S317, D7S820, D16S539, CSF1PO, PENTAD, VWA,D8S1179, TPOX, FGA) was performed using the PowerPlexÒ16System kit (Promega Corp., Madison, WI) (1) (PE-Biosystems,Foster City, CA) following the manufacturer’s protocol. The amplified products were separated and detected using the ABI PrismÒ310 genetic analyzer (Applied Biosystems, Foster City, CA) usingthe ABI Prism GeneScan analysis software version 3.1 and Geno-typer DNA fragment analysis software version 2.5.2 (2).The data were analyzed using Power-Stats (3). Allele frequenciesand other relevant information from analysis of the 15 STR lociare given in Table 1. The complete dataset is available to any inter-ested researcher upon request, or can be downloaded from thefollowing World Wide Web site: http://www.fcv.unlp.edu.ar/info-general/publicaciones/pdf/supplemtary.pdf.