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dc.contributor.author
Rossi, Soledad Paola
dc.contributor.author
Matzkin, Maria Eugenia
dc.contributor.author
Terradas, Claudio
dc.contributor.author
Ponzio, Roberto
dc.contributor.author
Puigdomenech, Elisa
dc.contributor.author
Levalle, Oscar
dc.contributor.author
Calandra, Ricardo Saul
dc.contributor.author
Frungieri, Monica Beatriz
dc.date.available
2016-12-20T20:10:56Z
dc.date.issued
2012-08
dc.identifier.citation
Rossi, Soledad Paola; Matzkin, Maria Eugenia; Terradas, Claudio; Ponzio, Roberto; Puigdomenech, Elisa; et al.; New insights into melatonin signaling in hamster Leydig cells; Academic Press Inc Elsevier Science; General And Comparative Endocrinology; 178; 1; 8-2012; 153-163
dc.identifier.issn
0016-6480
dc.identifier.uri
http://hdl.handle.net/11336/9852
dc.description.abstract
We have previously described that melatonin inhibits androgen production in hamster testes via melatonin subtype 1a (mel1a) receptors and the local corticotrophin-releasing hormone (CRH) system. This study attempted to determine the initial events of the melatonin/CRH signaling pathway. In Leydig cells from reproductively active Syrian hamsters, Western blotting, reverse transcription quantitative polymerase chain reaction (RT-qPCR) and a colorimetric assay demonstrated that melatonin and CRH activate tyrosine phosphatases and subsequently reduce the phosphorylation levels of extracellular signal-regulated kinase (erk) and c-jun N-terminal kinase (jnk), down-regulate the expression of c-jun, c-fos and steroidogenic acute regulatory (StAR), and inhibit the production of testosterone. These effects were prevented by a highly selective CRH antagonist, thus indicating that melatonin does not exert a direct role. Specific mitogen-activated protein kinase kinase (MEK) and jnk blockers inhibited expression of c-jun, c-fos, StAR and the production of testosterone, confirming that these are events triggered downstream of erk and jnk. In Leydig cells from photoperiodically regressed adult hamsters, CRH inhibited the production of androstane-3α,17β-diol (3α-diol), the main androgen produced, through the same signaling pathway. Testicular melatonin concentration was 3-4-fold higher in reproductively inactive hamsters than that detected in active animals. Since melatonin, CRH, and their receptors are present not only in hamster testes but also in testicular biopsies of infertile men, we can conjecture about the relevance of this previously uncharacterized pathway in human fertility disorders. In summary, our study identifies crucial intracellular events triggered by melatonin/CRH in the testis that lead to a down-regulation of the steroidogenic process.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Academic Press Inc Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Melatonin
dc.subject
Leydig Cells
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Intracellular Signalingi
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Tyrosine Phosphatases
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Testosterone
dc.subject.classification
Bioquímica y Biología Molecular
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
New insights into melatonin signaling in hamster Leydig cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-22T21:21:02Z
dc.identifier.eissn
1095-6840
dc.journal.volume
178
dc.journal.number
1
dc.journal.pagination
153-163
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Rossi, Soledad Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Matzkin, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Terradas, Claudio. Universidad de Buenos Aires. Facultad de Medicina; Argentina
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Fil: Ponzio, Roberto. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Puigdomenech, Elisa. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Levalle, Oscar. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.description.fil
Fil: Calandra, Ricardo Saul. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
dc.description.fil
Fil: Frungieri, Monica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina
dc.journal.title
General And Comparative Endocrinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.ygcen.2012.04.031
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0016648012001955
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