Artículo
Interdependence between EGFR and Phosphatases Spatially Established by Vesicular Dynamics Generates a Growth Factor Sensing and Responding Network
Stanoev, Angel; Mhamane, Amit; Schuermann, Klaus C.; Grecco, Hernan Edgardo
; Stallaert, Wayne; Baumdick, Martin; Brüggemann, Yannick; Joshi, Maitreyi S.; Roda Navarro, Pedro; Fengler, Sven; Stockert, Rabea; Roßmannek, Lisaweta; Luig, Jutta; Koseska, Aneta; Bastiaens, Philippe I.H.
Fecha de publicación:
09/2018
Editorial:
Cell Press
Revista:
Cell Systems
ISSN:
2405-4712
e-ISSN:
2405-4720
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The proto-oncogenic epidermal growth factor receptor (EGFR) is a tyrosine kinase whose sensitivity to growth factors and signal duration determines cellular behavior. We resolve how EGFR's response to epidermal growth factor (EGF) originates from dynamically established recursive interactions with spatially organized protein tyrosine phosphatases (PTPs). Reciprocal genetic PTP perturbations enabled identification of receptor-like PTPRG/J at the plasma membrane and ER-associated PTPN2 as the major EGFR dephosphorylating activities. Imaging spatial-temporal PTP reactivity revealed that vesicular trafficking establishes a spatially distributed negative feedback with PTPN2 that determines signal duration. On the other hand, single-cell dose-response analysis uncovered a reactive oxygen species-mediated toggle switch between autocatalytically activated monomeric EGFR and the tumor suppressor PTPRG that governs EGFR's sensitivity to EGF. Vesicular recycling of monomeric EGFR unifies the interactions with these PTPs on distinct membrane systems, dynamically generating a network architecture that can sense and respond to time-varying growth factor signals. Cells continuously respond to temporal changes of growth factors, but it is unclear how the surface receptor tyrosine kinase EGFR senses and translates these changes. We identify three protein tyrosine phosphatases localized on the plasma membrane and the ER that together do more than erase the phosphorylation signal written by this receptor. We show that vesicular recycling unifies the recursive interactions of these phosphatases with autocatalytically activated EGFR, thereby enabling responsiveness to time-varying EGF stimuli.
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Articulos(IFIBA)
Articulos de INST.DE FISICA DE BUENOS AIRES
Articulos de INST.DE FISICA DE BUENOS AIRES
Citación
Stanoev, Angel; Mhamane, Amit; Schuermann, Klaus C.; Grecco, Hernan Edgardo; Stallaert, Wayne; et al.; Interdependence between EGFR and Phosphatases Spatially Established by Vesicular Dynamics Generates a Growth Factor Sensing and Responding Network; Cell Press; Cell Systems; 7; 3; 9-2018; 295-309.e11
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