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dc.contributor.author
Bolognese, Belén
dc.contributor.author
Nerli, Bibiana Beatriz
dc.contributor.author
Picó, Guillermo Alfredo
dc.date.available
2020-01-20T18:10:36Z
dc.date.issued
2005-01
dc.identifier.citation
Bolognese, Belén; Nerli, Bibiana Beatriz; Picó, Guillermo Alfredo; Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification; Elsevier Science; Journal of Chromatography B; 814; 2; 1-2005; 347-353
dc.identifier.issn
0378-4347
dc.identifier.uri
http://hdl.handle.net/11336/95223
dc.description.abstract
In this study, the effect of several factors that govern the partitioning behaviour of three model proteins, such as bovine serum albumin, lysozyme and trypsin was analysed in a two-phase system formed by maltodextrin and a copolymer of ethylene and propylene oxides. The protein partition coefficient (K r) showed to be very sensitive to temperature changes, protein molecular weight, pH medium and the lyotropic ion presence. The phase diagram obtained for these novel polymer-polymer two-phase systems shows two phases with high polymer concentrations. The maltodextrin is enriched in the bottom phase while the copolymer of ethylene and propylene oxides is found in the upper phase. Since this copolymer is thermoreactive, the upper phase can be removed and heated above the copolymer's cloud point resulting in the formation of a new two-phase system with a lower water phase, containing the target protein and an upper copolymer-rich phase. Our results show that systems formed by maltodextrin and a copolymer of ethylene and propylene oxides may be considered as an interesting alternative to be used in protein purification due to their low cost, and also because they offer a viable solution to problems of polymer removal and recycling.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ETHYLENE OXIDE PROPYLENE OXIDE RANDOM COPOLYMER
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MALTODEXTRIN
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PARTITIONING
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TEMPERATURE-INDUCED TWO-PHASE SYSTEM
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Application of the aqueous two-phase systems of ethylene and propylene oxide copolymer-maltodextrin for protein purification
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-01-20T17:27:38Z
dc.identifier.eissn
1570-0232
dc.journal.volume
814
dc.journal.number
2
dc.journal.pagination
347-353
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Bolognese, Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.description.fil
Fil: Nerli, Bibiana Beatriz. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
dc.description.fil
Fil: Picó, Guillermo Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
dc.journal.title
Journal of Chromatography B
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1570023204008669
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.jchromb.2004.10.057
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