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dc.contributor.author
Pereira Da Costa, Javier Hernán  
dc.contributor.author
Rodríguez, Gustavo Rubén  
dc.contributor.author
Picardi, Liliana Amelia  
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Zorzoli, Roxana  
dc.contributor.author
Pratta, Guillermo Raúl  
dc.date.available
2019-11-28T22:29:42Z  
dc.date.issued
2018-02  
dc.identifier.citation
Pereira Da Costa, Javier Hernán; Rodríguez, Gustavo Rubén; Picardi, Liliana Amelia; Zorzoli, Roxana; Pratta, Guillermo Raúl; Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life; Elsevier Science; Scientia Horticulturae; 229; 2-2018; 125-131  
dc.identifier.issn
0304-4238  
dc.identifier.uri
http://hdl.handle.net/11336/90859  
dc.description.abstract
The ripening stage at harvest time determines the tomato fruit quality. After the fruit achieves its maximum size several metabolic changes of typically climacteric fruits are produced. Two cultivated genotypes of Solanum lycopersicum (Caimanta and 804627), with normal and altered fruit ripening, respectively and two accession, LA1385 of S. lycopersicum var. cerasiforme and LA722 of S. pimpinellifolium, with genes that prolong fruit shelf life, were tested to: 1) characterize and make a comparatively analysis for the transcriptome at different fruit ripening stages in genotypes that differ in fruit shelf life by cDNA-AFLP; and 2) provide further insight into the relationship between the extreme phenotypic differences for ripening among the genotypes through changes at transcriptomic level. Fruits at the breaker stage (B) were evaluated for fruit weight, firmness and fruit shelf life. The elapsed days between mature green (MG) and breaker stages Days (MG-B) as well as the elapsed days between B and red ripe (RR) stages Days (B-RR) were recorded. Comparison among ripening stages showed a great polymorphism related to the changes in gene expression. For all genotypes the transition from B to RR stages had higher polymorphism than the transition from MG to B. It was observed a great genetic variability for the phenotypic traits in agreement with the changes of gene expression. Moreover, it was observed that the transcriptome expression profiles in the initial and intermediate stages during ripening (MG and B) are more important to characterize genotypes. The wild species which have long shelf life do not show as drastic changes in gene expression as the cultivar with altered ripening that carrythe nor gene. These results suggest that the expressed or silenced genes could be involved, in some way, in the determination of the phenotypic traits evaluated in this study.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
CDNA-AFLP  
dc.subject
FRUIT RIPENING PROCESS  
dc.subject
GENETIC VARIABILITY  
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NOR  
dc.subject
SOLANUM LYCOPERSICUM  
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SOLANUM PIMPINELLIFOLIUM  
dc.subject.classification
Horticultura, Viticultura  
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Agricultura, Silvicultura y Pesca  
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CIENCIAS AGRÍCOLAS  
dc.title
Genome-wide expression analysis at three fruit ripening stages for tomato genotypes differing in fruit shelf life  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2019-10-23T20:48:06Z  
dc.journal.volume
229  
dc.journal.pagination
125-131  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Pereira Da Costa, Javier Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina  
dc.description.fil
Fil: Rodríguez, Gustavo Rubén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina  
dc.description.fil
Fil: Picardi, Liliana Amelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina  
dc.description.fil
Fil: Zorzoli, Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina  
dc.description.fil
Fil: Pratta, Guillermo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina  
dc.journal.title
Scientia Horticulturae  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.scienta.2017.11.004