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dc.contributor.author
Astrada, Soledad
dc.contributor.author
Gomez, Yolanda
dc.contributor.author
Barrera Guisasola, Exequiel Ernesto
dc.contributor.author
Obal, Gonzalo
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Pritsch, Otto
dc.contributor.author
Pantano, Sergio
dc.contributor.author
Vallespí, Maribel G.
dc.contributor.author
Bollati Fogolín, Mariela
dc.date.available
2019-11-28T19:49:21Z
dc.date.issued
2016-11
dc.identifier.citation
Astrada, Soledad; Gomez, Yolanda; Barrera Guisasola, Exequiel Ernesto; Obal, Gonzalo; Pritsch, Otto; et al.; Comparative analysis reveals amino acids critical for anticancer activity of peptide CIGB-552; John Wiley & Sons Ltd; Journal Of Peptide Science; 22; 11-12; 11-2016; 711-722
dc.identifier.issn
1075-2617
dc.identifier.uri
http://hdl.handle.net/11336/90821
dc.description.abstract
Because of resistance development by cancer cells against current anticancer drugs, there is a considerable interest in developing novel antitumor agents. We have previously demonstrated that CIGB-552, a novel cell-penetrating synthetic peptide, was effective in reducing tumor size and increasing lifespan in tumor-bearing mice. Studies of protein–peptide interactions have shown that COMMD1 protein is a major mediator of CIGB-552 antitumor activity. Furthermore, a typical serine-protease degradation pattern for CIGB-552 in BALB/c mice serum was identified, yielding peptides which differ from CIGB-552 in size and physical properties. In the present study, we show the results obtained from a comparative analysis between CIGB-552 and its main metabolites regarding physicochemical properties, cellular internalization, and their capability to elicit apoptosis in MCF-7 cells. None of the analyzed metabolites proved to be as effective as CIGB-552 in promoting apoptosis in MCF-7. Taking into account these results, it seemed important to examine their cell-penetrating capacity and interaction with COMMD1. We show that internalization, a lipid binding-dependent process, is impaired as well as metabolite–COMMD1 interaction, key component of the apoptotic mechanism. Altogether, our results suggest that features conferred by the amino acid sequence are decisive for CIGB-552 biological activity, turning it into the minimal functional unit.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
John Wiley & Sons Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AMINO ACIDS
dc.subject
APOPTOSIS
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CELL PENETRATING PEPTIDE
dc.subject
COMMD1
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Comparative analysis reveals amino acids critical for anticancer activity of peptide CIGB-552
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-11-21T17:36:01Z
dc.journal.volume
22
dc.journal.number
11-12
dc.journal.pagination
711-722
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Astrada, Soledad. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Gomez, Yolanda. Center For Genetic Engineering And Biotechnology; Cuba
dc.description.fil
Fil: Barrera Guisasola, Exequiel Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Pasteur de Montevideo. Laboratorio de Simuladores Biomoleculares; Uruguay
dc.description.fil
Fil: Obal, Gonzalo. Instituto Pasteur de Montevideo; Uruguay. Universidad de la República; Uruguay
dc.description.fil
Fil: Pritsch, Otto. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Pantano, Sergio. Instituto Pasteur de Montevideo; Uruguay
dc.description.fil
Fil: Vallespí, Maribel G.. Center For Genetic Engineering And Biotechnology; Cuba
dc.description.fil
Fil: Bollati Fogolín, Mariela. Instituto Pasteur de Montevideo; Uruguay
dc.journal.title
Journal Of Peptide Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/psc.2934
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/psc.2934
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