Mostrar el registro sencillo del ítem
dc.contributor.author
Grillo, Claudia Alejandra
dc.contributor.author
Dulout, Fernando Noel
dc.contributor.author
Güerci, Alba Mabel
dc.date.available
2019-08-26T20:34:10Z
dc.date.issued
2009-02
dc.identifier.citation
Grillo, Claudia Alejandra; Dulout, Fernando Noel; Güerci, Alba Mabel; Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model; Taylor & Francis; International Journal Of Radiation Biology; 85; 2; 2-2009; 159-166
dc.identifier.issn
0955-3002
dc.identifier.uri
http://hdl.handle.net/11336/82168
dc.description.abstract
Purpose: The present study was designed to evaluate the effects of sequential exposures to low doses of gamma-radiation that induce a radioadaptive response to a later high-dose of radiation in CHO-K1 cells. Materials and methods: Cells were cultured in four dilution cycles and grown to confluency. Radiation treatment was performed once per cycle with 0.1 Gy gamma-rays. After the last radiation period (chronic radiation) the culture was irradiated with a higher dose (1 Gy). Each cell culture was immediately divided into two fractions: one of them was used to carry out the comet assay and the other for the structural chromosome aberration test. In the first fraction, genotoxic damage was evaluated by degree of damage in 300 cells per experimental point. The second assay was performed with 400 cells per treatment. The statistical analysis was carried out using the 2-test. Results: Results from these assays demonstrated a genotoxic effect for both the adaptive and acute treatments (p0.001). The comet assay showed a significant increase in damage for the combined treatment when compared with 1 Gy treatment (p0.001). The frequency of chromosomal aberrations (CA) was lower for the combined treatment than for that using the highest radiation dose. Conclusions: These results suggest the possible induction of a radioadaptive response after the sequential exposure to very low doses of radiation. The finding of decreased cytogenetic damage after one cell cycle and not immediately after radiation could indicate the eventual potentiation of repair mechanisms.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Comet Assay
dc.subject
Radioadaptive Response
dc.subject
Structural Chromosome Aberration Test
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Evaluation of radioadaptive response induced in CHO-K1 cells in a non-traditional model
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-07-02T16:25:27Z
dc.identifier.eissn
1362-3095
dc.journal.volume
85
dc.journal.number
2
dc.journal.pagination
159-166
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Grillo, Claudia Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Dulout, Fernando Noel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Güerci, Alba Mabel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Investigaciones en Genética Básica y Aplicada; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
International Journal Of Radiation Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/09553000902740143
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/09553000902740143
Archivos asociados