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dc.contributor.author
Papaiakovou, Marina
dc.contributor.author
Pilotte, Nils
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Grant, Jessica R.
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Traub, Rebecca J.
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Llewellyn, Stacey
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McCarthy, James S.
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Krolewiecki, Alejandro Javier
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Cimino, Rubén Oscar
dc.contributor.author
Mejia, Rojelio
dc.contributor.author
Williams, Steven A.
dc.date.available
2019-02-05T21:10:17Z
dc.date.issued
2017-07
dc.identifier.citation
Papaiakovou, Marina; Pilotte, Nils; Grant, Jessica R.; Traub, Rebecca J.; Llewellyn, Stacey; et al.; A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool; Public Library of Science; PLoS Neglected Tropical Diseases; 11; 7; 7-2017; 1-11
dc.identifier.issn
1935-2735
dc.identifier.uri
http://hdl.handle.net/11336/69477
dc.description.abstract
Background: Molecular-based surveys have indicated that Ancylostoma ceylanicum, a zoonotic hookworm, is likely the second most prevalent hookworm species infecting humans in Asia. Most current PCR-based diagnostic options for the detection of Ancylostoma species target the Internal Transcribed Spacer (ITS) regions of the ribosomal gene cluster. These regions possess a considerable degree of conservation among the species of this genus and this conservation can lead to the misidentification of infecting species or require additional labor for accurate species-level determination. We have developed a novel, real-time PCR-based assay for the sensitive and species-specific detection of A. ceylanicum that targets a non-coding, highly repetitive genomic DNA element. Comparative testing of this PCR assay with an assay that targets ITS sequences was conducted on field-collected samples from Argentina and Timor-Leste to provide further evidence of the sensitivity and species-specificity of this assay. Methods/Principal findings: A previously described platform for the design of primers/probe targeting non-coding highly repetitive regions was used for the development of this novel assay. The assay’s limits of detection (sensitivity) and cross-reactivity with other soil-transmitted helminth species (specificity) were assessed with real-time PCR experiments. The assay was successfully used to identify infections caused by A. ceylanicum that were previously only identified to the genus level as Ancylostoma spp. when analyzed using other published primer-probe pairings. Further proof of sensitive, species-specific detection was provided using a published, semi-nested restriction fragment length polymorphism-PCR assay that differentiates between Ancylostoma species. Conclusions/Significance: Due to the close proximity of people and domestic/wild animals in many regions of the world, the potential for zoonotic infections is substantial. Sensitive tools enabling the screening for different soil-transmitted helminth infections are essential to the success of mass deworming efforts and facilitate the appropriate interpretation of data. This study describes a novel, species-specific, real-time PCR-based assay for the detection of A. ceylanicum that will help to address the need for such tools in integrated STH deworming programs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ancylostoma Ceylanicum
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Human
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Salta
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Argentina
dc.subject.classification
Salud Ocupacional
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
A novel, species-specific, real-time PCR assay for the detection of the emerging zoonotic parasite Ancylostoma ceylanicum in human stool
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-01-02T19:22:51Z
dc.journal.volume
11
dc.journal.number
7
dc.journal.pagination
1-11
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Papaiakovou, Marina. Smith College; Estados Unidos
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Fil: Pilotte, Nils. Smith College; Estados Unidos. University of Massachussets; Estados Unidos
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Fil: Grant, Jessica R.. Smith College; Estados Unidos
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Fil: Traub, Rebecca J.. University of Melbourne; Australia
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Fil: Llewellyn, Stacey. Qimr Berghofer Medical Research Institute; Australia
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Fil: McCarthy, James S.. Qimr Berghofer Medical Research Institute; Australia
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Fil: Krolewiecki, Alejandro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Cimino, Rubén Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta; Argentina. Universidad Nacional de Salta. Sede Regional Orán. Instituto de Investigación de Enfermedades Tropicales; Argentina
dc.description.fil
Fil: Mejia, Rojelio. Baylor College Of Medicine; Estados Unidos
dc.description.fil
Fil: Williams, Steven A.. Smith College; Estados Unidos. University of Massachussets; Estados Unidos
dc.journal.title
PLoS Neglected Tropical Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1371/journal.pntd.0005734
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005734
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