Artículo
Production, partial purification and characterization of α-l-rhamnosidase from Penicillium ulaiense
Rajal, Verónica Beatriz
; Cid, Alicia Graciela
; Von Ellenrieder, Guillermo Rodolfo
; Cuevas, Carlos Mario
Fecha de publicación:
06/2009
Editorial:
Springer
Revista:
World Journal of Microbiology
ISSN:
0959-3993
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Penicillium ulaiense is a post-harvest pathogenic fungus that attacks citrus fruits. The objective of this work was to study this microorganism as an α-l-rhamnosidase producer and to characterize it from P. ulaiense. The enzyme under study is used for different applications in food and beverage industries. α-l-Rhamnosidase was produced in a stirred-batch reactor using rhamnose as the main carbon source. The kinetic parameters for the growth of the fungi and for the enzyme production were calculated from the experimental values. A method for partial purification, including (NH4) 2SO4 precipitation, incubation at pH 12 and DEAE-sepharose chromatography yielded an enzyme with very low β-glucosidase activity. The pH and temperature optima were 5.0 and 60°C, respectively. The Michaelis-Menten constants for the hydrolysis of p-nitrophenyl-α-l- rhamnoside were V max = 26 ± 4 IU ml-1 and K m = 11 ± 2 mM. The enzyme showed good thermostability up to 60°C and good operational stability in white wine. Co2+ affected positively the activity; EDTA, Mn2+, Mg2+, dithiotreitol and Cu2+ reduced the activity by different amounts, and Hg 2+ completely inhibited the enzyme. The enzyme showed more activity on p-nitrophenyl-α-l-rhamnoside than on naringin. According to these results, this enzyme has potential for use in the food and pharmacy industries since P. ulaiense does not produce mycotoxins. © 2009 Springer Science+Business Media B.V.
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Articulos(INIQUI)
Articulos de INST.DE INVEST.PARA LA INDUSTRIA QUIMICA (I)
Articulos de INST.DE INVEST.PARA LA INDUSTRIA QUIMICA (I)
Citación
Rajal, Verónica Beatriz; Cid, Alicia Graciela; Von Ellenrieder, Guillermo Rodolfo; Cuevas, Carlos Mario; Production, partial purification and characterization of α-l-rhamnosidase from Penicillium ulaiense; Springer; World Journal of Microbiology; 25; 6; 6-2009; 1025-1033
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