Mostrar el registro sencillo del ítem
dc.contributor.author
Soria, Elio Andres
dc.contributor.author
Perez, Roberto Daniel
dc.contributor.author
Queralt, Ignasi
dc.contributor.author
Pérez, Carlos A.
dc.contributor.author
Bongiovanni, Guillermina Azucena
dc.date.available
2018-09-03T14:57:56Z
dc.date.issued
2017-01
dc.identifier.citation
Soria, Elio Andres; Perez, Roberto Daniel; Queralt, Ignasi; Pérez, Carlos A.; Bongiovanni, Guillermina Azucena; Immunotoxicological effects of arsenic bioaccumulation on spatial metallomics and cellular enzyme response in the spleen of male Wistar rats after oral intake; Elsevier Ireland; Toxicology Letters; 266; 1-2017; 65-73
dc.identifier.issn
0378-4274
dc.identifier.uri
http://hdl.handle.net/11336/58050
dc.description.abstract
Arsenic (As) is a worldwide environmental contaminant, which compromises immunity and causes various associated disorders. To further investigate its immunotoxicity, male Wistar rats were exposed to 100 ppm of sodium arsenite (inorganic AsIII) in drinking water for 2 months. Given that metals are significant immune regulators, their content and distribution were analysed in spleen tissues, to then evaluate subsequent changes of redox enzyme responses in spleen parenchyma cells (splenocytes). X-ray fluorescence spectrometry demonstrated As accumulation in both white and red pulps (p < 0.005), and As-related pulp-dependent modifications of the content of Cu, Ca, Zn and Fe (p < 0.01). Correlational path analysis revealed direct effects of As on their spatial distribution (Cu: −0.76, Ca: −0.61, Zn: 0.38; p < 0.02). As-exposed splenocytes showed ɣ-glutamyltranspeptidase inhibition, peroxidase induction, and variable responses of nitric oxide synthase (p < 0.05). Concanavalin A-treated splenocytes (T cell mitogen) were more susceptible in vitro to these As-related enzymatic changes than those treated with lipopolysaccharide (B cell mitogen) (p < 0.05). The study thus established the impact of As bioaccumulation on metallic spatial homeostasis in the spleen, and then identified enzymatic dysfunctions in splenocytes. This suggested that arsenic disrupts biometal-dependent immune pathways and redox homeostasis, with mitogen exposure modifying the toxicological response.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
IMMUNODEFICIENCY
dc.subject
OXIDATIVE STRESS
dc.subject
SODIUM ARSENITE
dc.subject
X-RAY FLUORESCENCE SPECTROMETRY
dc.subject.classification
Otras Ciencias Biológicas
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Immunotoxicological effects of arsenic bioaccumulation on spatial metallomics and cellular enzyme response in the spleen of male Wistar rats after oral intake
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-29T18:40:30Z
dc.journal.volume
266
dc.journal.pagination
65-73
dc.journal.pais
Irlanda
dc.journal.ciudad
Dublin
dc.description.fil
Fil: Soria, Elio Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
dc.description.fil
Fil: Perez, Roberto Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
dc.description.fil
Fil: Queralt, Ignasi. Institute of Environmental Assessment and Water Research; España
dc.description.fil
Fil: Pérez, Carlos A.. Laboratorio Nacional de Luz Sincrotron; Brasil
dc.description.fil
Fil: Bongiovanni, Guillermina Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas. Universidad Nacional del Comahue. Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas; Argentina
dc.journal.title
Toxicology Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0378427416333550
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.toxlet.2016.12.014
Archivos asociados