Mostrar el registro sencillo del ítem

dc.contributor.author
Indarte, Martín  
dc.contributor.author
Lazza, Cristian Martin  
dc.contributor.author
Assis, Diego  
dc.contributor.author
Caffini, Nestor Oscar  
dc.contributor.author
Juliano, María A.  
dc.contributor.author
Avilés, Francesc X.  
dc.contributor.author
Daura, Xavier  
dc.contributor.author
Lopez, Laura Maria Isabel  
dc.contributor.author
Trejo, Sebastian Alejandro  
dc.date.available
2018-08-22T18:53:27Z  
dc.date.issued
2017-02  
dc.identifier.citation
Indarte, Martín; Lazza, Cristian Martin; Assis, Diego; Caffini, Nestor Oscar; Juliano, María A.; et al.; A Bowman–Birk protease inhibitor purified, cloned, sequenced and characterized from the seeds of Maclura pomifera (Raf.) Schneid; Springer; Planta; 245; 2; 2-2017; 343-353  
dc.identifier.issn
0032-0935  
dc.identifier.uri
http://hdl.handle.net/11336/56658  
dc.description.abstract
Main conclusion: A new BBI-type protease inhibitor with remarkable structural characteristics was purified, cloned, and sequenced from seeds of Maclura pomifera, a dicotyledonous plant belonging to the Moraceae family. In this work, we report a Bowman–Birk inhibitor (BBI) isolated, purified, cloned, and characterized from Maclura pomifera seeds (MpBBI), the first of this type from a species belonging to Moraceae family. MpBBI was purified to homogeneity by RP-HPLC, total RNA was extracted from seeds of M. pomifera, and the 3′RACE-PCR method was applied to obtain the cDNA, which was cloned and sequenced. Peptide mass fingerprinting (PMF) analysis showed correspondence between the in silico-translated protein and MpBBI, confirming that it corresponds to a new plant protease inhibitor. The obtained cDNA encoded a polypeptide of 65 residues and possesses 10 cysteine residues, with molecular mass of 7379.27, pI 6.10, and extinction molar coefficient of 9105�M−1�cm−1. MpBBI inhibits strongly trypsin with Ki in the 10−10 M range and was stable in a wide array of pH and extreme temperatures. MpBBI comparative modeling was applied to gain insight into its 3D structure and highlighted some distinguishing features: (1) two non-identical loops, (2) loop 1 (CEEESRC) is completely different from any known BBI, and (3) the amount of disulphide bonds is also different from any reported BBI from dicot plants.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Bbi-Type Protease Inhibitor  
dc.subject
Cloning  
dc.subject
Homology Modeling  
dc.subject
Loop  
dc.subject
Three-Dimensional Structure  
dc.subject
Trypsin Inhibition  
dc.subject.classification
Otras Ciencias Biológicas  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
A Bowman–Birk protease inhibitor purified, cloned, sequenced and characterized from the seeds of Maclura pomifera (Raf.) Schneid  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-08-21T19:10:14Z  
dc.identifier.eissn
1432-2048  
dc.journal.volume
245  
dc.journal.number
2  
dc.journal.pagination
343-353  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlín  
dc.description.fil
Fil: Indarte, Martín. Phusis Therapeutics; Estados Unidos  
dc.description.fil
Fil: Lazza, Cristian Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina  
dc.description.fil
Fil: Assis, Diego. Universidade de Sao Paulo; Brasil  
dc.description.fil
Fil: Caffini, Nestor Oscar. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigación de Proteínas Vegetales; Argentina  
dc.description.fil
Fil: Juliano, María A.. Universitat Autònoma de Barcelona; España  
dc.description.fil
Fil: Avilés, Francesc X.. Universitat Autònoma de Barcelona; España  
dc.description.fil
Fil: Daura, Xavier. Universitat Autònoma de Barcelona; España  
dc.description.fil
Fil: Lopez, Laura Maria Isabel. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo del Cuero. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Centíficas. Centro de Investigación y Desarrollo del Cuero; Argentina. Universidad Nacional Arturo Jauretche; Argentina  
dc.description.fil
Fil: Trejo, Sebastian Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto Multidisciplinario de Biología Celular. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Instituto Multidisciplinario de Biología Celular. Universidad Nacional de La Plata. Instituto Multidisciplinario de Biología Celular; Argentina  
dc.journal.title
Planta  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00425-016-2611-6  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00425-016-2611-6