Mostrar el registro sencillo del ítem
dc.contributor.author
Mantegazza, Adriana Rita
dc.contributor.author
Savina, Ariel
dc.contributor.author
Vermeulen, Elba Monica
dc.contributor.author
Pérez, Laura
dc.contributor.author
Geffner, Jorge Raúl
dc.contributor.author
Hermine, Olivier
dc.contributor.author
Rosenzweig, Sergio D.
dc.contributor.author
Faure, Florence
dc.contributor.author
Amigorena, Sebastián
dc.date.available
2018-08-16T18:35:18Z
dc.date.issued
2008-12
dc.identifier.citation
Mantegazza, Adriana Rita; Savina, Ariel; Vermeulen, Elba Monica; Pérez, Laura; Geffner, Jorge Raúl; et al.; NADPH oxidase controls phagosomal pH and antigen cross-presentation in human dendritic cells; American Society of Hematology; Blood; 112; 12; 12-2008; 4712-4722
dc.identifier.issn
0006-4971
dc.identifier.uri
http://hdl.handle.net/11336/55972
dc.description.abstract
The phagocyte NADPH oxidase (NOX2) is critical for the bactericidal activity of phagocytic cells and plays a major role in innate immunity. We showed recently that NOX2 activity in mouse dendritic cells (DCs) prevents acidification of phagosomes, promoting antigen cross-presentation. In order to investigate the role of NOX2 in the regulation of the phagosomal pH in human DCs, we analyzed the production of reactive oxygen species (ROS) and the phagosomal/endosomal pH in monocyte-derived DCs and macrophages (MØs) from healthy donors or patients with chronic granulomatous disease (CGD). As expected, we found that human MØs acidify their phagosomes more efficiently than human DCs. Accordingly, the expression of the vacuolar proton ATPase (V-H+-ATPase) was higher in MØs than in DCs. Phagosomal ROS production, however, was also higher in MØs than in DCs, due to higher levels of gp91 phoxexpression and recruitment to phagosomes. In contrast, in the absence of active NOX2, the phagosomal and endosomal pH decreased. Both in the presence of a NOX2 inhibitor and in DCs derived from patients with CGD, the cross-presentation of 2 model tumor antigens was impaired. We conclude that NOX2 activity participates in the regulation of the phagosomal and endosomal pH in human DCs, and is required for efficient antigen cross-presentation. © 2008 by The American Society of Hematology.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Hematology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject.classification
Inmunología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
NADPH oxidase controls phagosomal pH and antigen cross-presentation in human dendritic cells
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-14T13:58:38Z
dc.journal.volume
112
dc.journal.number
12
dc.journal.pagination
4712-4722
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Mantegazza, Adriana Rita. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut Curie; Francia
dc.description.fil
Fil: Savina, Ariel. Institut Curie; Francia
dc.description.fil
Fil: Vermeulen, Elba Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
dc.description.fil
Fil: Pérez, Laura. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
dc.description.fil
Fil: Geffner, Jorge Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires. Instituto de Investigaciones Hematológicas "Mariano R. Castex"; Argentina
dc.description.fil
Fil: Hermine, Olivier. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Rosenzweig, Sergio D.. Gobierno de la Ciudad de Buenos Aires. Hospital de Pediatría "Juan P. Garrahan"; Argentina
dc.description.fil
Fil: Faure, Florence. Institut Curie; Francia
dc.description.fil
Fil: Amigorena, Sebastián. Institut Curie; Francia
dc.journal.title
Blood
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1182/blood-2008-01-134791
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.bloodjournal.org/content/112/12/4712
Archivos asociados