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dc.contributor.author
Landoni, Verónica Inés
dc.contributor.author
de Campos Nebel, Ildefonso Marcelo
dc.contributor.author
Schierloh, Luis Pablo
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Calatayud, Cecilia Alicia
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Fernández, Gabriela Cristina
dc.contributor.author
Ramos, Maria Victoria
dc.contributor.author
Rearte, María Bárbara
dc.contributor.author
Palermo, Marina Sandra
dc.contributor.author
Isturiz, Martín Amadeo
dc.date.available
2018-08-14T14:18:54Z
dc.date.issued
2010-03
dc.identifier.citation
Landoni, Verónica Inés; de Campos Nebel, Ildefonso Marcelo; Schierloh, Luis Pablo; Calatayud, Cecilia Alicia; Fernández, Gabriela Cristina; et al.; Shiga toxin 1-induced inflammatory response in lipopolysaccharide- sensitized astrocytes is mediated by endogenous tumor necrosis factor alpha; American Society for Microbiology; Infection and Immunity; 78; 3; 3-2010; 1193-1201
dc.identifier.issn
0019-9567
dc.identifier.uri
http://hdl.handle.net/11336/55332
dc.description.abstract
Hemolytic-uremic syndrome (HUS) is generally caused by Shiga toxin (Stx)-producing Escherichia coli. Endothelial dysfunction mediated by Stx is a central aspect in HUS development. However, inflammatory mediators such as bacterial lipopolysaccharide (LPS) and polymorphonuclear neutrophils (PMN) contribute to HUS pathophysiology by potentiating Stx effects. Acute renal failure is the main feature of HUS, but in severe cases, patients can develop neurological complications, which are usually associated with death. Although the mechanisms of neurological damage remain uncertain, alterations of the blood-brain barrier associated with brain endothelial injury is clear. Astrocytes (ASTs) are the most abundant inflammatory cells of the brain that modulate the normal function of brain endothelium and neurons. The aim of this study was to evaluate the effects of Stx type 1 (Stx1) alone or in combination with LPS in ASTs. Although Stx1 induced a weak inflammatory response, pretreatment with LPS sensitized ASTs to Stx1-mediated effects. Moreover, LPS increased the level of expression of the Stx receptor and its internalization. An early inflammatory response, characterized by the release of tumor necrosis factor alpha (TNF-α) and nitric oxide and PMN-chemoattractant activity, was induced by Stx1 in LPS-sensitized ASTs, whereas activation, evidenced by higher levels of glial fibrillary acid protein and cell death, was induced later. Furthermore, increased adhesion and PMN-mediated cytotoxicity were observed after Stx1 treatment in LPS-sensitized ASTs. These effects were dependent on NF-κB activation or AST-derived TNF-α. Our results suggest that TNF-α is a pivotal effector molecule that amplifies Stx1 effects on LPS-sensitized ASTs, contributing to brain inflammation and leading to endothelial and neuronal injury. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Astrocytes
dc.subject
Shiga Toxin 1
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Hemolytic-Uremic Syndrome
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Brain Inflammation
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Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Shiga toxin 1-induced inflammatory response in lipopolysaccharide- sensitized astrocytes is mediated by endogenous tumor necrosis factor alpha
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-08-08T14:08:07Z
dc.journal.volume
78
dc.journal.number
3
dc.journal.pagination
1193-1201
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Bethesda
dc.description.fil
Fil: Landoni, Verónica Inés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: de Campos Nebel, Ildefonso Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Schierloh, Luis Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Calatayud, Cecilia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina
dc.description.fil
Fil: Fernández, Gabriela Cristina. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Ramos, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Rearte, María Bárbara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil
Fil: Isturiz, Martín Amadeo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.journal.title
Infection and Immunity
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1128/IAI.00932-09
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://iai.asm.org/content/78/3/1193
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