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dc.contributor.author
Chaud, Luciana C. S.  
dc.contributor.author
Lario, Luciana Daniela  
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Bonugli Santos, Rafaella C.  
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Sette, Lara D.  
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Pessoa Junior, Adalberto  
dc.contributor.author
Felipe, Maria das Graças de A.  
dc.date.available
2018-07-23T19:43:31Z  
dc.date.issued
2016-12  
dc.identifier.citation
Chaud, Luciana C. S.; Lario, Luciana Daniela; Bonugli Santos, Rafaella C.; Sette, Lara D.; Pessoa Junior, Adalberto; et al.; Improvement in extracellular protease production by the marine antarctic yeast Rhodotorula mucilaginosa L7; Elsevier Science; New Biotechnology; 33; 6; 12-2016; 807-814  
dc.identifier.issn
1871-6784  
dc.identifier.uri
http://hdl.handle.net/11336/52889  
dc.description.abstract
Microorganisms from extreme and restrictive eco systems, such as the Antarctic continent, are of great interest due to their ability to synthesize products of commercial value. Among these, enzymes from psychrotolerant and psychrophilic microorganisms offer potential economical benefits due to their high activity at low and moderate temperatures. The cold adapted yeast Rhodotorula mucilaginosa L7 was selected out of 97 yeasts isolated from Antarctica as having the highest extracellular proteolytic activity in preliminary tests. The present study was aimed at evaluating the effects of nutrient composition (peptone, rice bran extract, ammonium sulfate, sodium chloride) and physicochemical parameters (temperature and pH) on its proteolytic activity. A 26−2 fractional factorial design experiment followed by a central composite design (CCD 23) was performed to optimize the culture conditions and improve the extracellular proteolytic activity. The results indicated that the presence of peptone in the medium was the most influential factor in protease production. Enzymatic activity was enhanced by the interaction between low glucose and peptone concentrations. The optimization of culture conditions with the aid of mathematical modeling enabled a c. 45% increase in proteolytic activity and at the same time reduced the amount of glucose and peptone required for the culture. Thus culture conditions established in this work may be employed in the biotechnological production of this protease.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Elsevier Science  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Antarctica Yeast  
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Nutritional Supplementation  
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Protease  
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Rhodotorula Mucilaginosa  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Improvement in extracellular protease production by the marine antarctic yeast Rhodotorula mucilaginosa L7  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2018-07-23T12:55:35Z  
dc.journal.volume
33  
dc.journal.number
6  
dc.journal.pagination
807-814  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Chaud, Luciana C. S.. Universidade de Sao Paulo; Brasil  
dc.description.fil
Fil: Lario, Luciana Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidade de Sao Paulo; Brasil  
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Fil: Bonugli Santos, Rafaella C.. Federal University of Latin American Integration; Brasil  
dc.description.fil
Fil: Sette, Lara D.. Universidade de Sao Paulo; Brasil  
dc.description.fil
Fil: Pessoa Junior, Adalberto. Universidade de Sao Paulo; Brasil  
dc.description.fil
Fil: Felipe, Maria das Graças de A.. Universidade de Sao Paulo; Brasil  
dc.journal.title
New Biotechnology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1016/j.nbt.2016.07.016  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1871678416323330