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dc.contributor.author
Ledda, Angelo
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Gonzalez, Marina Cecilia
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Gulfo, José
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Díaz Ludovico, Ivo
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Ramella, Nahuel
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Toledo, Juan Domingo
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Garda, Horacio Alberto
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Grasa, María del Mar(senior author)
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Esteve, Montserrat
dc.date.available
2018-06-14T21:54:35Z
dc.date.issued
2016-07
dc.identifier.citation
Ledda, Angelo; Gonzalez, Marina Cecilia; Gulfo, José; Díaz Ludovico, Ivo; Ramella, Nahuel; et al.; Decreased OxLDL uptake and cholesterol efflux in THP1 cells elicited by cortisol and by cortisone through 11β-hydroxysteroid dehydrogenase type 1; Elsevier Ireland; Atherosclerosis; 250; 7-2016; 84-94
dc.identifier.issn
0021-9150
dc.identifier.uri
http://hdl.handle.net/11336/48744
dc.description.abstract
Background and aims: Data about glucocorticoids role in the development of atherosclerosis are controversial showing different effects in human than in experimental animal models. Atherosclerosis is the result of a chronic inflammatory response to an injured endothelium where an uncontrolled uptake of OxLDL by macrophages triggers the development of foam cells, the main component of fatty streaks in atherosclerotic plaque. There are few data about the direct effect of glucocorticoids in macrophages of atherosclerotic plaque. The aim of the study was to elucidate the role of glucocorticoids in the development of foam cells in atherosclerosis initiation. Methods: For this purpose we used THP1 cells differentiated to macrophages with phorbol esters and incubated with OxLDL alone or with cortisol or cortisone. THP1 cells were also incubated with cortisone plus an inhibitor of 11β-hydroxysteroid dehydrogenase 1 (11βHSD1) activity to determine the role of this enzyme on glucocorticoid action in this process. Results: Ours results showed that cortisol and cortisone decreased significantly the inflammation promoted by OxLDL, and also diminished the expression of genes involved in influx and efflux of cholesterol resulting in a reduced lipid accumulation. Likewise cortisol and cortisone decreased 11βHSD1 expression in THP1 cells. The presence of the inhibitor of 11βHSD1 abolished all the effects elicited by cortisone. Conclusion: Our results indicate a direct effect of glucocorticoids on macrophages braking atherosclerosis initiation, reducing pro-inflammatory markers and OxLDL uptake and cholesterol re-esterification, but also inhibiting cholesterol output. These effects appear to be mediated, at least in part, by 11βHSD1 activity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland
dc.relation
https://doi.org/10.1016/j.dib.2016.05.046
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
11β-Hydroxysteroid Dehydrogenase 1
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11β-Hydroxysteroid Dehydrogenase 2
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Cortisol
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Cortisone
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Foam Cells
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Thp1 Macrophages
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Foam Cells
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Decreased OxLDL uptake and cholesterol efflux in THP1 cells elicited by cortisol and by cortisone through 11β-hydroxysteroid dehydrogenase type 1
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-06-14T19:05:54Z
dc.journal.volume
250
dc.journal.pagination
84-94
dc.journal.pais
Irlanda
dc.journal.ciudad
Shannon
dc.description.fil
Fil: Ledda, Angelo. Universidad de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España
dc.description.fil
Fil: Gonzalez, Marina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Gulfo, José. Universidad de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España
dc.description.fil
Fil: Díaz Ludovico, Ivo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Ramella, Nahuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Toledo, Juan Domingo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Garda, Horacio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; Argentina
dc.description.fil
Fil: Grasa, María del Mar(senior author). Universidad de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España
dc.description.fil
Fil: Esteve, Montserrat. Universidad de Barcelona; España. Universidad Carlos III de Madrid. Instituto de Salud; España
dc.journal.title
Atherosclerosis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.atherosclerosis.2016.04.020
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0021915016301514
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