Artículo
Mesenchymal stromal cells improve cardiac function and left ventricular remodeling in a heart transplantation model
Montanari, Sonia; Dayan, Victor; Yannarelli, Gustavo Gabriel
; Billia, Filio; Viswanathan, Sowmya; Connelly, Kim A.; Keating, Armand
Fecha de publicación:
11/2015
Editorial:
Elsevier Science Inc
Revista:
Journal of Heart and Lung Transplantation
ISSN:
1053-2498
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Ischemia/reperfusion (I/R) injury is an inevitable consequence of organ transplantation and a major determinant of patient and graft survival in heart transplantation. Bone marrow–mesenchymal stromal cell (BM-MSC) treatment is a potentially effective cell therapy for cardiac disease. We investigated the effects of intravenous delivery of BM-MSCs in the acute phase post-transplant in a heterotopic heart transplantation (HHT) model associated with I/R injury. Methods: Hearts of wild-type Lewis (WT LEW) rats were harvested and transplanted heterotopically into the necks of recipient WT LEW rats. Forty-eight hours after HHT, BM-MSCs were injected intravenously into animals in the experimental group, whereas controls received normal saline (NS). Results: Eight days after BM-MSC injection, fractional shortening of transplanted hearts was significantly higher and left ventricular systolic diameter was lower in the BM-MSC group compared with controls, whereas no differences were found 28 days after infusion. A reduction in ventricular remodeling and cardiac fibrosis was observed by histochemical analysis and confirmed by cardiac magnetic resonance imaging in the BM-MSC group. The perivascular stromal cells’ density and the number of capillaries were increased whereas the number of apoptotic cells decreased significantly in transplanted hearts in the BM-MSC group compared with the NS group. Conclusions: We showed early improvement in cardiac function and subsequent enhanced ventricular remodeling, reduced cardiac fibrosis, augmented neo-vascularization and decreased cardiomyocyte apoptosis of the transplanted heart in a heterotopic transplantation model after intravenous infusion of BM-derived MSCs. Our data suggest that clinical studies with BM-MSCs are warranted to understand their effects on cardiac graft and transplant recipient survival.
Palabras clave:
Mesenchymal Stromal Cells
,
Heart Transplantation
,
Remodeling
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Montanari, Sonia; Dayan, Victor; Yannarelli, Gustavo Gabriel; Billia, Filio; Viswanathan, Sowmya; et al.; Mesenchymal stromal cells improve cardiac function and left ventricular remodeling in a heart transplantation model; Elsevier Science Inc; Journal of Heart and Lung Transplantation; 34; 11; 11-2015; 1481-1488
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