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dc.contributor.author
Martínez Esteso, María José
dc.contributor.author
Sellés Marchart, Susana
dc.contributor.author
Lijavetzky, Diego Claudio
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Pedreño, María Ángeles
dc.contributor.author
Bru Martínez, Roque
dc.date.available
2018-04-12T19:43:08Z
dc.date.issued
2011-05
dc.identifier.citation
Martínez Esteso, María José; Sellés Marchart, Susana; Lijavetzky, Diego Claudio; Pedreño, María Ángeles; Bru Martínez, Roque; A DIGE-based quantitative proteomic analysis of grape berry flesh development and ripening reveals key events in sugar and organic acid metabolism; Oxford University Press; Journal of Experimental Botany; 62; 8; 5-2011; 2521-2569
dc.identifier.issn
0022-0957
dc.identifier.uri
http://hdl.handle.net/11336/41901
dc.description.abstract
Grapevine (Vitis vinifera L.) is an economically important fruit crop. The berry components that determine the final quality traits of both table and wine grapes, such as sugars, acids, flavours, anthocyanins, tannins, etc., are synthesized or accumulated along the different grape berry development stages. Thus, correlating the proteomic profiles with the biochemical and physiological changes known to occur in the grape berries along development is of paramount importance to advance in the understanding the ripening process. We report the developmental analysis of V. vinifera cv. Muscat d’Hamburg berries at protein level. We undertook a top-down proteomic approach based on differential in-gel electrophoresis (DIGE) followed by tandem mass spectrometry to determine differentially accumulated proteins. The green development (from fruit set to pre-veraison) and the ripening (from veraison to full ripening) were analysed as independent experiments. We have identified and quantified 154 and 61 deregulated proteins in green and ripening phases, respectively. Two key points in development at the protein change level have been determined: end of green development and beginning of ripening. An additional DIGE experiment was performed comparing fully ripe berries from two vintages in order to detect seasonal changes. As few spots changed we have concluded that protein changes detected in this study are developmental-specific. The role of amount of identified proteins is discussed in relation to grape berry development and to quality traits. The well known shift of imported sucrose fate at the beginning of ripening from accumulation of organic acid (malate) to hexoses (glucose and fructose) was well correlated with a switch between sucrose synthase and soluble acid invertase.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Oxford University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Berry Development
dc.subject
Proteomic Analysis
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A DIGE-based quantitative proteomic analysis of grape berry flesh development and ripening reveals key events in sugar and organic acid metabolism
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-04-05T18:16:47Z
dc.journal.volume
62
dc.journal.number
8
dc.journal.pagination
2521-2569
dc.journal.pais
Reino Unido
dc.journal.ciudad
Oxford
dc.description.fil
Fil: Martínez Esteso, María José. Universidad de Alicante; España
dc.description.fil
Fil: Sellés Marchart, Susana. Universidad de Alicante; España
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Fil: Lijavetzky, Diego Claudio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Consejo Superior de Investigaciones Científicas; España
dc.description.fil
Fil: Pedreño, María Ángeles. Universidad de Murcia; España
dc.description.fil
Fil: Bru Martínez, Roque. Universidad de Alicante; España
dc.journal.title
Journal of Experimental Botany
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/jxb/erq434
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/62/8/2521/471500
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