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dc.contributor.author
Calleros Basilio, Laura
dc.contributor.author
Cortés, María Alicia
dc.contributor.author
Garcia Jerez, Andrea
dc.contributor.author
Luengo Rodríguez, Alicia
dc.contributor.author
Orozco Agudo, Ana
dc.contributor.author
Valdivielso, José Manuel
dc.contributor.author
Rodríguez Puyol, Diego
dc.contributor.author
Rodríguez Puyol, Manuel
dc.date.available
2018-03-21T17:56:38Z
dc.date.issued
2016-12
dc.identifier.citation
Calleros Basilio, Laura; Cortés, María Alicia; Garcia Jerez, Andrea; Luengo Rodríguez, Alicia; Orozco Agudo, Ana; et al.; Quality Assurance of Samples and Processes in the Spanish Renal Research Network (REDinREN) Biobank; Mary Ann Liebert; Biopreservation and Biobanking; 14; 6; 12-2016; 499-510
dc.identifier.issn
1947-5543
dc.identifier.uri
http://hdl.handle.net/11336/39533
dc.description.abstract
Background: Biobanks are useful platforms to build bridges between basic, translational, and clinical research and clinical care. They are repositories of high-quality human biological samples ideal for evaluating their histological characteristics and also their genome, transcriptome, and proteome. The Spanish Renal Research Network Biobank contains more than 76,500 well-preserved frozen samples of a wide variety of kidney diseases, collected from 5450 patients seen by over 70 nephrology services throughout the Spanish territory. Objective: To determine and to report the results of the quality control of samples and processes conducted in our biobank, implemented in accordance with the requirements of the ISO 9001:2008 international standard. Study Design: Two types of quality controls were performed: (1) systematic, that is, measurement of viable peripheral blood mononuclear cells (PBMCs) obtained and purity of nucleic acids and (2) ad-hoc, that is, viability of thawed PBMC, DNA extraction process reproducibility, and the integrity and functionality of nucleic acids, implemented on a routine basis. Methods and Results: PBMC isolation by Ficoll yielded reproducible results and its cryopreserved viability was >90%. Acceptable A260/A280 ratios were obtained for the vast majority of the DNA (n = 2328) and RNA (n = 78) samples analyzed. DNA integrity was demonstrated by agarose gels and by β-globulin gene polymerase chain reaction (PCR) amplification of 1327 and 989 bp fragments. DNA of acceptable quality had at least three bands of β-globulin amplified obtained (n = 26/30). RNA integrity number (RIN) determinations obtained RIN numbers ≥7 (n = 87/96). The amplifiability of nucleic acids was confirmed by qPCR and RT-qPCR of β-actin and GAPDH genes. Long storage or delayed processing time did not affect the quality of the samples analyzed. The processes of DNA extraction also yielded reproducible results. Conclusions: These results clearly indicate that our PBMC, DNA, and RNA stored samples meet the required quality standards to be used for biomedical research, ensuring their long-term preservation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Mary Ann Liebert
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Biobank
dc.subject
Chronic Kidney Disease
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Iso 9001:2008
dc.subject
Sample Quality Control
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Inmunología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Quality Assurance of Samples and Processes in the Spanish Renal Research Network (REDinREN) Biobank
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-20T13:13:03Z
dc.journal.volume
14
dc.journal.number
6
dc.journal.pagination
499-510
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Calleros Basilio, Laura. Ministerio de Economía, Industria y Competitividad. Instituto de Salud Carlos III; España. Universidad de Alcalá; España
dc.description.fil
Fil: Cortés, María Alicia. Universidad Nacional del Nordeste. Facultad de Medicina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Garcia Jerez, Andrea. Ministerio de Economía, Industria y Competitividad. Instituto de Salud Carlos III; España. Universidad de Alcalá; España
dc.description.fil
Fil: Luengo Rodríguez, Alicia. Ministerio de Economía, Industria y Competitividad. Instituto de Salud Carlos III; España. Universidad de Alcalá; España
dc.description.fil
Fil: Orozco Agudo, Ana. Ministerio de Economía, Industria y Competitividad. Instituto de Salud Carlos III; España. Universidad de Alcalá; España
dc.description.fil
Fil: Valdivielso, José Manuel. Ministerio de Economía, Industria y Competitividad. Instituto de Salud Carlos III; España. Universidad de Lleida. Instituto de Recerca Biomédica; España
dc.description.fil
Fil: Rodríguez Puyol, Diego. Ministerio de Economía, Industria y Competitividad. Instituto de Salud Carlos III; España. Hospital Universitario Príncipe de Asturias; España
dc.description.fil
Fil: Rodríguez Puyol, Manuel. Ministerio de Economía, Industria y Competitividad. Instituto de Salud Carlos III; España. Universidad de Alcalá; España
dc.journal.title
Biopreservation and Biobanking
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.liebertpub.com/doi/abs/10.1089/bio.2015.0095
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1089/bio.2015.0095
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