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dc.contributor.author
Judkowski, Valeria A.
dc.contributor.author
Santos, Radleigh G.
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Acevedo, Gonzalo Raúl
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Giulianotti, Marc A.
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Appel, Jon R.
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Longhi, Silvia Andrea
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Gomez, Karina Andrea
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Pinilla, Clemencia
dc.date.available
2016-02-01T20:28:14Z
dc.date.issued
2015-03
dc.identifier.citation
Judkowski, Valeria A.; Santos, Radleigh G.; Acevedo, Gonzalo Raúl; Giulianotti, Marc A.; Appel, Jon R.; et al.; Antigen discovery for the identification of vaccine candidates and biomarkers using a T cell driven approach in combination with positional scanning peptide libraries; Elsevier; Procedia in Vaccinology; 9; 3-2015; 91-95
dc.identifier.issn
1877-282X
dc.identifier.uri
http://hdl.handle.net/11336/3943
dc.description.abstract
The prevention and treatment of infectious diseases is highly dependent on the availability of reliable diagnostic tests and protective or therapeutic vaccines. There also exists an urgent need to develop reliable biomarkers to monitor treatment success and to predict disease progression from asymptomatic to symptomatic disease in several disease scenarios. The elucidation of the disease-relevant antigens that elicit the protective immune responses is critical and required for the development of biomarkers, diagnostics, and vaccines. However; one of the main obstacles to the study of antigen specificity in human T cells is their low frequency in PBMC samples. To overcome this problem we have implemented strategies to generate memory T cell libraries and clones specific to the pathogen of interest. Due to the fact that memory T cells represent a repository of the human T cell response to infection, examination of their antigen specificity can efficiently reveal immunogenic and relevant antigens involved in the in vivo response to infection or vaccines. To examine the specificity of the memory T cells we use an unbiased collection of antigens together with an in silico analysis, namely positional scanning based biometrical analysis. Here we present a summary of our approach and ongoing work on the development of strategies for the culture of memory T cells from patients with Chagas disease.While most studies focus on the identification of vaccine candidates using preselected immunogenic proteins derived from animal models or by or bioinformatics prediction, here we present an innovative approach that directly examines the specificity of the memory response following infection or immunization in humans.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Antigen Discovery
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Vaccines Candidate
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Human T Cells
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T Cell Specificity
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Antigen discovery for the identification of vaccine candidates and biomarkers using a T cell driven approach in combination with positional scanning peptide libraries
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.volume
9
dc.journal.pagination
91-95
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.conicet.avisoEditorial
This is an open access article under the CC BY-NC-ND license
dc.description.fil
Fil: Judkowski, Valeria A.. Torrey Pines Institute for Molecular Studies; Estados Unidos
dc.description.fil
Fil: Santos, Radleigh G.. Torrey Pines Institute for Molecular Studies; Estados Unidos
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Fil: Acevedo, Gonzalo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
dc.description.fil
Fil: Giulianotti, Marc A.. Torrey Pines Institute for Molecular Studies; Estados Unidos
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Fil: Appel, Jon R.. Torrey Pines Institute for Molecular Studies; Estados Unidos
dc.description.fil
Fil: Longhi, Silvia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
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Fil: Gomez, Karina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular; Argentina
dc.description.fil
Fil: Pinilla, Clemencia. Torrey Pines Institute for Molecular Studies; Estados Unidos
dc.journal.title
Procedia in Vaccinology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1877282X15000144
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.provac.2015.05.013
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info:eu-repo/semantics/altIdentifier/issn/1877-282X
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