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dc.contributor.author
Fernandez Lopez, Laura
dc.contributor.author
Rueda, Nazoly
dc.contributor.author
Bartolome Cabrero, Rocio
dc.contributor.author
Rodríguez, María Daniela
dc.contributor.author
Albuquerque, Tiago. L.
dc.contributor.author
dos Santos, Jose C. S.
dc.contributor.author
Barbosa, Oveimar
dc.contributor.author
Fernandez-Lafuente, Roberto
dc.date.available
2018-03-15T21:10:57Z
dc.date.issued
2016-01
dc.identifier.citation
Fernandez Lopez, Laura; Rueda, Nazoly; Bartolome Cabrero, Rocio; Rodríguez, María Daniela; Albuquerque, Tiago. L.; et al.; Improved immobilization and stabilization of lipase from Rhizomucor miehei on octyl-glyoxyl agarose beads by using CaCl2; Elsevier; Process Biochemistry; 51; 1; 1-2016; 48-52
dc.identifier.issn
1359-5113
dc.identifier.uri
http://hdl.handle.net/11336/39049
dc.description.abstract
A strategy to stabilize octyl (OC)-Rhizomucor miehei lipase (RML) and OC-Candida rugosa lipase (CRL) at pH 10 is necessary to take full advantage of the immobilization of these enzymes on OC-glyoxyl (OCGLX) supports. CaCl2 and MnCl2 have been reported to stabilize OC-RM and OC-CRL at pH 5.0 and 7.0. After screening different buffers, 5 mM CaCl2 was found to be fully soluble in Gly at pH 10. OC-RML was 15 folds stabilized at this pH value by CaCl2, while OC-CRL was not. This salt was used in the preparation of octyl-glyoxyl (OCGLX)-RML, permitting to maintain almost unaltered the OC-RML activity that was 3 fold higher than that of the free. This preparation was 30% and 3 folds more stable in thermal or acetonitrile inactivations respectively than the standard OCGLX one. The stabilizing effect of CaCl2 and MnCl2 on the OCGLX preparations was studied. These salts stabilized both OCGLX-RML preparations, although the one prepared using Ca2+ during the covalent attachment was more stabilized than the standard one by the presence of Ca2+, even 7-8 folds in the presence of aceonitrile. Thus, this additive permits to recover an OCGLX-RML preparation more stable and active than the standard protocol.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cations
dc.subject
Covalent Immobilization
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Lipase Interfacial Activation
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Lipase Stabilization
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Octyl-Glyoxyl
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Stabilizing Additives
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Improved immobilization and stabilization of lipase from Rhizomucor miehei on octyl-glyoxyl agarose beads by using CaCl2
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2018-03-09T18:44:05Z
dc.identifier.eissn
1873-3298
dc.journal.volume
51
dc.journal.number
1
dc.journal.pagination
48-52
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Fernandez Lopez, Laura. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
dc.description.fil
Fil: Rueda, Nazoly. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España. Universidad Industrial Santander; Colombia
dc.description.fil
Fil: Bartolome Cabrero, Rocio. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
dc.description.fil
Fil: Rodríguez, María Daniela. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Albuquerque, Tiago. L.. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España. Universidade Federal Do Ceará. Departamento de Engenharia Química; Brasil
dc.description.fil
Fil: dos Santos, Jose C. S.. Universidade Federal Do Ceará. Departamento de Engenharia Química; Brasil. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
dc.description.fil
Fil: Barbosa, Oveimar. Universidad del Tolima. Facultad de Ciencias Exactas. Departamento de Química; Colombia
dc.description.fil
Fil: Fernandez-Lafuente, Roberto. Consejo Superior de Investigaciones Científicas. Instituto de Catálisis y Petroleoquímica; España
dc.journal.title
Process Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.procbio.2015.11.015
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