Artículo
Levans production by Gluconacetobacter diazotrophicus
Fecha de publicación:
04/2013
Editorial:
Univ Catolica de Valparaiso
Revista:
Electronic Journal Of Biotechnology
ISSN:
0717-3458
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Growth of Gluconacetobacterdiazotrophicuswith glucose as carbon an energy source has been extensively studied. However, there are no reports in the literature describing growth of G.diazotrophicusin cultures containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. Biomass, levans, gluconic acid andketogluconic acids production and levansucrase activity were determined in cultures with different sucrose concentration and nitrogen sources. Results: The biomass production was maximal in cultures containing 100 gL-1 sucrose and inorganic nitrogen. Gluconic acid production was observed under all conditions tested, at levels up to 9 gL-1 in cultures with sucrose excess and biological N2-fixation (BNF). Ketogluconic acids were detectable only in cultures with sucrose excess and supplemented with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 gL-1 of sucrose and BNF, concomitant with a significant expression of extracellular levansucrase. Conclusions: Ours results not only describe some unknown aspects of G. diazotrophicus physiology, but open up the possibility of developing a technology oflevans production by this organism using culture media with sucrose (or some cheaper substitute, like molasses) and without the addition of any N-source because of its ability of fixing atmospheric N2.
Palabras clave:
Levans
,
Fos
,
Levansucrase
,
Gluconacetobacter Diazotrophicus
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Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Citación
Molinari, Maria Laura; Boiardi, Jose Luis; Levans production by Gluconacetobacter diazotrophicus; Univ Catolica de Valparaiso; Electronic Journal Of Biotechnology; 4-2013
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