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dc.contributor.author
Persia, Andres
dc.contributor.author
Mariani, María Laura Lucía
dc.contributor.author
Fogal, Teresa Hilda
dc.contributor.author
Penissi, Alicia Beatriz
dc.date.available
2018-01-02T19:35:46Z
dc.date.issued
2014-07
dc.identifier.citation
Persia, Andres; Mariani, María Laura Lucía; Fogal, Teresa Hilda; Penissi, Alicia Beatriz; Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways; Elsevier Gmbh; Phytomedicine; 21; 11; 7-2014; 1400-1405
dc.identifier.issn
0944-7113
dc.identifier.uri
http://hdl.handle.net/11336/32037
dc.description.abstract
The aim of the present work was to determine whether hydroxytyrosol and oleuropein, the major polyphenols in olives and olive oil, inhibit mast cell activation induced by immune and non-immune pathways. Purified peritoneal mast cells were preincubated in the presence of test compounds (hydroxytyrosol or oleuropein), before incubation with concanavalin A, compound 48/80 or calcium ionophore A23187. Dose-response and time-dependence studies were carried out. Comparative studies with sodium chromoglycate, a classical mast cell stabilizer, were also made. The supernatants and pellets after incubations were used for determination of β-hexosaminidase content by colorimetric reaction. The percentage of β-hexosaminidase release in each tube was calculated, which was taken as a measure of mast cell activation. Other samples of cell pellets were used for cell viability studies by the trypan blue dye exclusion test, or fixed for light and electron microscopy. Biochemical and morphological findings of the present study showed for the first time that hydroxytyrosol and oleuropein inhibit the degranulation of mast cells induced by immune and non-immune pathways. These results suggest that olive polyphenols, particularly hydroxytyrosol and oleuropein, may provide insights into the development of tools useful in preventing and treating mast cell-mediated disorders.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Gmbh
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hydroxytyrosol
dc.subject
Oleuropein
dc.subject
Olive Oil
dc.subject
Mast Cell
dc.subject
Olea Europaea
dc.subject
Β-Hexosaminidase
dc.subject
Degranulation
dc.subject.classification
Farmacología y Farmacia
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Hydroxytyrosol and Oleuropein Inhibit Mast Cell Degranulation Induced by Immune and Non-Immune Pathways
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-12-29T14:11:33Z
dc.journal.volume
21
dc.journal.number
11
dc.journal.pagination
1400-1405
dc.journal.pais
Alemania
dc.description.fil
Fil: Persia, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Mariani, María Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Fogal, Teresa Hilda. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.description.fil
Fil: Penissi, Alicia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos. Universidad Nacional de Cuyo. Facultad de Cienicas Médicas. Instituto de Histología y Embriología de Mendoza Dr. Mario H. Burgos; Argentina
dc.journal.title
Phytomedicine
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.phymed.2014.05.010
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711314002499
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