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dc.contributor.author
Ritta, Monica Nora
dc.contributor.author
Bas, Diana Ester
dc.contributor.author
Tartaglione, C. M.
dc.date.available
2017-11-26T03:40:28Z
dc.date.issued
2004-02
dc.identifier.citation
Ritta, Monica Nora; Bas, Diana Ester; Tartaglione, C. M.; In vitro effect of gamma-aminobutyric acid on bovine spermatozoa capacitation; Wiley-liss, Inc; Molecular Reproduction and Development; 67; 4; 2-2004; 478-486
dc.identifier.issn
1040-452X
dc.identifier.uri
http://hdl.handle.net/11336/29121
dc.description.abstract
Sperm capacitation is defined as the maturational changes that render a sperm competent for fertilization and occurs in the female reproductive tract. Identification of the factor/s that regulate sperm capacitation would allow the understanding of these phenomena. Among these factors, gamma-aminobutyric acid (GABA) has recently become as a putative modulator of sperm function. The aim of this study was to explore the presence of a GABAergic regulation of bovine sperm capacitation as well as the possible intracellular mechanisms involved. GABA was detected in fresh semen by a sensitive radioreceptor assay (spermatozoa, 0.064 +/- 0.003 nmoles/10(6) cells; seminal plasma, 23.21 +/- 1.16 nmoles/ml). Scatchard analysis of [(3)H]-muscimol binding to sperm membranes yielded a linear plot consistent with a single population of binding sites (K(d) = 3.87 nM, B(max) = 417 fmol/mg prot.). [(3)H]-muscimol specific binding to sperm membranes was significantly inhibited by the GABA A receptor (GABA A-R) antagonist bicuculline and by the agonists muscimol and isoguvacine. Addition of GABA to the incubation medium resulted in a concentration-dependent increase in the percentage of capacitated spermatozoa (chlortetracycline assay). We observed a significant increment on intracellular calcium and cyclic 3',5' adenosine monophosphate (cAMP) concentrations induced by GABA, being the cation influx abolished when the cell suspensions were coincubated with the antagonists bicuculline or picrotoxin. It is concluded that GABA induces sperm capacitation through an intracellular mechanism dependent on calcium influx and cAMP accumulation mediated by a specific GABA A-R.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley-liss, Inc
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Spermatazoa
dc.subject
Bovine
dc.subject
Gaba
dc.subject
Capacitation
dc.subject
Clortetracycline
dc.subject.classification
Otras Biotecnología Agropecuaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
In vitro effect of gamma-aminobutyric acid on bovine spermatozoa capacitation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-11-16T15:13:30Z
dc.journal.volume
67
dc.journal.number
4
dc.journal.pagination
478-486
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Ritta, Monica Nora. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Bas, Diana Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina. Universidad Nacional de Lomas de Zamora; Argentina
dc.description.fil
Fil: Tartaglione, C. M.. Universidad Nacional de Lomas de Zamora. Facultad de Ciencias Agrarias; Argentina
dc.journal.title
Molecular Reproduction and Development
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/mrd.20038/full
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/mrd.20038
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/14991740
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