Matrix solid-phase dispersion: a simple and fast technique for the determination of phenolic compounds in olive oil by liquid chromatography

Abstract

A methodology was developed using matrix solid phase dispersion, together with liquid chromatography with multiple wavelength detector for the determination of twenty phenolic compounds in olive oil samples. Under optimized conditions, the analytes were extracted using 0.5 g of olive oil, 1.0 g of Florisil as sorbent and methanol:water (80:20 acidified with formic acid 0.5% (v/v)) as eluting solvent. The proposed methodology provided detection and quantification limits in the ranges of 0.023 - 0.750 and 0.075 - 2.500 mg kg-1, respectively. The RSDs resulting from the analysis of 6 replicates of 0.5 g of sample pool containing 2.5 mg kg-1 phenolic compounds were ranged between 2.1% and 14.8%.Considering matrix-matched calibration as quantification technique, the average recoveries ranged from 74.8% to 95.0%, with relative standard deviations between 1.5% and 9.3%.The developed methodology was applied for the determination of phenolic compounds in nine olive oils produced in Argentina, identifying seventeen analytes at concentrations above detectable levels.