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dc.contributor.author
Alippi, Adriana Mónica
dc.contributor.author
López, Ana Claudia
dc.contributor.author
Balatti, Pedro Alberto
dc.date.available
2023-04-11T14:28:41Z
dc.date.issued
2012-08
dc.identifier.citation
Alippi, Adriana Mónica; López, Ana Claudia; Balatti, Pedro Alberto; Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina; Springer; European Journal of Plant Pathology; 134; 2; 8-2012; 415-430
dc.identifier.issn
0929-1873
dc.identifier.uri
http://hdl.handle.net/11336/193256
dc.description.abstract
The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to be Agrobacterium since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730 bp partial sequence that codes for virulence genes within the virC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to Agrobacterium bv. 1 (n = 33) or Agrobacterium bv. 2 (n = 31) only fourteen were Agrobacterium rubi. A representative isolate of each of these three groups was further identified by sequencing the approximately 400 bp 16SrDNA. We concluded that Vaccinium plants are particularly susceptible to Agrobacterium bv. 1, Agrobacterium bv. 2, and also to Agrobacterium rubi. To our knowledge this is the first survey of Agrobacterium affecting blueberries in Argentina.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AGROBACTERIUM RHIZOGENES
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AGROBACTERIUM RUBI
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AGROBACTERIUM TUMEFACIENS
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BIOVARS
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BLUEBERRIES
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CROWN GALL DISEASE
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DIVERSITY
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PCR
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VACCINIUM CORYMBOSSUM
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-29T17:29:14Z
dc.journal.volume
134
dc.journal.number
2
dc.journal.pagination
415-430
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Alippi, Adriana Mónica. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
dc.description.fil
Fil: López, Ana Claudia. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
dc.description.fil
Fil: Balatti, Pedro Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
dc.journal.title
European Journal of Plant Pathology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10658-012-0001-x
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s10658-012-0001-x
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