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dc.contributor.author
Angelicola, María Virginia
dc.contributor.author
Viñarta, Silvana Carolina
dc.contributor.author
Aybar, Manuel Javier
dc.contributor.author
Castellanos, Lucia Ines
dc.date.available
2023-02-13T14:59:45Z
dc.date.issued
2018
dc.identifier.citation
Effect of temperature on growth and lipid production of the cold-adapted Rhodotorula glutinis strains; International Specialized Symposium on Yeasts 34; Bariloche; Argentina; 2018; 1-3
dc.identifier.uri
http://hdl.handle.net/11336/187749
dc.description.abstract
Cold-adapted oleaginous yeasts are excellent lipid-producers and they are considered as a potential source of triglycerides (TAG) for the biodiesel synthesis. The lipogenesis is influenced by temperature. This work evaluated the effect of different temperatures on the growth and lipids accumulation by two strains of Rhodotorula glutinis (R4 and R48). Yeasts were cultured (168 h, 250 rpm) at 15, 20 and 25°C in nitrogen-limited glucose-based medium (MI). An increase of biomass, lipid production and total lipids content was observed along the culture time for the yeasts at the three incubation temperatures. The highest biomass (11.48-13.53 g L-1) was yielded after 72 h at 20 and 25°C and remained constant until the end of the culture. At 15°C the growth was slower for both strains. Effects of temperature on lipid production and accumulation were more pronounced in R48 strain. The highest values of these parameters were reached at 20° C (5.64 g L-1; 45.74) and 25°C (5.48 g L-1; 49.15%) after 120 h of culture. While at 15 °C results were lower. R4 was less affected by temperature and significant differences were not observed. The higher lipid production (5.68-7.00 g L-1) and lipids accumulation (48.02-53.00%) were yielded after 120 h. The presence of TAG in microbial lipids was determined qualitatively using thin layer chromatography (TLC). The results showed that these yeasts are able to synthesize TAG under the culture conditions tested. Qualitatively, there was an increase in the production of TAG (larger size and density of spots) with temperature. The fatty acids profile determined by GC-FID is suitable for biodiesel synthesis. R4 and R48 are interesting yeasts for microbial oils production and they are capable to grow and produce lipids between 15 and 25°C.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Universidad Nacional del Comahue
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Temperature
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Cold-adapted strains
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Rhodotorula glutinis
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Lipid production
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Effect of temperature on growth and lipid production of the cold-adapted Rhodotorula glutinis strains
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-02-06T13:54:07Z
dc.journal.pagination
1-3
dc.journal.pais
Argentina
dc.journal.ciudad
Bariloche
dc.description.fil
Fil: Angelicola, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Viñarta, Silvana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Aybar, Manuel Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.barilocheeventos.com/34o-international-specialized-symposium-on-yeasts/
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Internacional
dc.type.subtype
Simposio
dc.description.nombreEvento
International Specialized Symposium on Yeasts 34
dc.date.evento
2018-10
dc.description.ciudadEvento
Bariloche
dc.description.paisEvento
Argentina
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Universidad Nacional del Comahue;
dc.description.institucionOrganizadora
Instituto Andino Patagónico de Tecnologías Biológicas y Geoambientales
dc.description.institucionOrganizadora
Instituto de Investigación y Desarrollo en Ingeniería de Procesos, Biotecnología y Energías Alternativas
dc.source.libro
Book of abstacts: International Specialized Symposium on Yeasts ISSY 34
dc.type
Simposio
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