Artículo
Rheological properties of a two phase lipid monolayer at the air/water interface: Effect of the composition of the mixture
Fecha de publicación:
03/2010
Editorial:
American Chemical Society
Revista:
Langmuir
ISSN:
0743-7463
e-ISSN:
1520-5827
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Many biologically relevant monolayers show coexistence of discrete domains of a long-range ordered condensed phase dispersed in a continuous, disordered, liquid-expanded phase. In this work, we determined the viscous and elastic components of the compressibility modulus and the shear viscosity of monolayers exhibiting phase coexistence with the aim at elucidating the contribution of each phase to the observed monolayer mechanical properties. To this purpose, mixed monolayers with different proportions of distearoylphosphatidylcholine (DSPC) and dimyristoylphosphatidylcholine (DMPC) were prepared and their rheological properties were analyzed. The relationship between the phase diagram of the mixture at 10 mN m-1 and the rheological properties was studied. We found that the monolayer shear viscosity is highly dependent on the presence of domains and on the domain density. In turn, the monolayer compressibility is only influenced by the presence of domains for high domain densities. For monolayers that look homogeneous on the micrometer scale (DSPC amount lower that 23 mol %), all the analyzed rheological properties remain similar to those observed for pure DMPC monolayers, indicating that in this proportion range the DSPC molecules contribute as DMPC to the surface rheology in spite of having hydrocarbon chains four carbons longer.
Palabras clave:
MEMBRANE ELECTROSTATICS
,
LIPID DOMAINS
,
SHEAR VISCOSITY
,
COMPRESIBILITY
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Identificadores
Colecciones
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Wilke, Natalia; Vega Mercado, Franco; Maggio, Bruno; Rheological properties of a two phase lipid monolayer at the air/water interface: Effect of the composition of the mixture; American Chemical Society; Langmuir; 26; 13; 3-2010; 11050-11059
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