Artículo
Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment
Fecha de publicación:
12/2005
Editorial:
Società Italiana di Istochimica
Revista:
European Journal of Histochemistry
ISSN:
1121-760X
e-ISSN:
2038-8306
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The gonadotropes, LH and FSH cells, were immunohistochemically identified in the pituitary pars distalis of the adult male viscacha (Lagostomus maximus maximus) using specific antibodies against hLHb and hFSHb with the streptavidin-biotin-peroxidase complex. The distribution, size and percentage immunopositive area of these cells were analyzed by image analysis in viscachas captured during the annual reproductive cycle and after the chronic administration of melatonin. The LHb and FSHb cells showed seasonal changes in the distribution, size and percentage immunopositive area. The LHb cells were found widely distributed throughout the pars distalis during the reproductive period, and they were found in the ventro-medial region in the pars distalis during the gonadal regression and gonadal recovery periods. The LHb cells reached the largest size and immunopositive area during the reproductive period and the smallest size and immunopositive area during the gonadal regression period. The FSHb cells were found in the ventromedial region during reproductive and gonadal regression periods. The FSHb cells were found widely distributed throughout the pars distalis during the gonadal recovery period when they showed the maximum percentage immunopositive area. A decrease in the size of LHb and FSHb cells was observed after the chronic administration of melatonin. Moreover, it produces a decrease in the immunopositive area occupied by the LHb cells but not in the immunopositive area occupied by the FSHb cells. Our results show great activity of LHb and FSHb cells in different moments of the annual reproductive cycle demonstrating that these cells do not secrete in parallel. Moreover, melatonin acts differentially on the activity of the gonadotrope cells.
Palabras clave:
PITUITARY
,
LH
,
FSH
,
IMMUNOHISTOCHEMISTRY
,
VISCACHA
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - SAN LUIS)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - SAN LUIS
Citación
Filippa, Veronica Palmira; Penissi, Alicia Beatriz; Mohamed, Fabian Heber; Seasonal variations of gonadotropins in the pars distalis male viscacha pituitary: Effect of chronic melatonin treatment; Società Italiana di Istochimica; European Journal of Histochemistry; 49; 3; 12-2005; 291-300
Compartir
Altmétricas