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dc.contributor.author
Luquita, A.
dc.contributor.author
Urli, L.
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Svetaz, M. J.
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Gennaro, Ana Maria
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Giorgietti, M.
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Pistone, G.
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Volpintesta, R.
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Palatnik, S.
dc.contributor.author
Rasia, M.
dc.date.available
2017-03-13T14:16:41Z
dc.date.issued
2010-12
dc.identifier.citation
Luquita, A.; Urli, L.; Svetaz, M. J.; Gennaro, Ana Maria; Giorgietti, M.; et al.; In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties; Biomed Central; Journal Of Biomedical Science; 17; 12-2010; 8-14
dc.identifier.issn
1021-7770
dc.identifier.uri
http://hdl.handle.net/11336/13757
dc.description.abstract
Background: Hyaluronic acid (HA) is present in many tissues; its presence in serum may be related to certaininflammatory conditions, tissue damage, sepsis, liver malfunction and some malignancies. In the present work, ourgoal was to investigate the significance of hyaluronic acid effect on erythrocyte flow properties. Therefore weperformed in vitro experiments incubating red blood cells (RBCs) with several HA concentrations. Afterwards, in orderto corroborate the pathophysiological significance of the results obtained, we replicated the in vitro experiment with exvivo RBCs from diagnosed rheumatoid arthritis (RA) patients, a serum HA-increasing pathology.Methods: Erythrocyte deformability (by filtration through nucleopore membranes) and erythrocyte aggregability (EA)were tested on blood from healthy donors additioned with purified HA. EA was measured by transmitted light andanalyzed with a mathematical model yielding two parameters, the aggregation rate and the size of the aggregates.Conformational changes of cytoskeleton proteins were estimated by electron paramagnetic resonance spectroscopy(EPR).Results: In vitro, erythrocytes treated with HA showed increased rigidity index (RI) and reduced aggregability, situationstrongly related to the rigidization of the membrane cytoskeleton triggered by HA, as shown by EPR results. Also, asignificant correlation (r: 0.77, p Conclusions: Our results lead us to postulate the hypothesis that HA interacts with the erythrocyte surface leading tomodifications in erythrocyte rheological and flow properties, both ex vivo and in vitro.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Biomed Central
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Hyaluronic Acid
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Erythrocyte Deformability
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Erythrocyte Aggregation
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Rheumatoid Arthritis
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-24T19:29:10Z
dc.journal.volume
17
dc.journal.pagination
8-14
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Luquita, A.. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Urli, L.. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Svetaz, M. J.. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Gennaro, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química (i); Argentina. Universidad Nacional del Litoral; Argentina
dc.description.fil
Fil: Giorgietti, M.. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Pistone, G.. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Volpintesta, R.. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Palatnik, S.. Universidad Nacional de Rosario; Argentina
dc.description.fil
Fil: Rasia, M.. Universidad Nacional de Rosario; Argentina
dc.journal.title
Journal Of Biomedical Science
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jbiomedsci.biomedcentral.com/articles/10.1186/1423-0127-17-8
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1186/1423-0127-17-8
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