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dc.contributor.author
Martínez Noël, Giselle María Astrid
dc.contributor.author
Nagaraj, Vinay Janthakahalli
dc.contributor.author
Caló, Gonzalo Federico
dc.contributor.author
Wiemken, Andrés S.
dc.contributor.author
Pontis, Horacio Guillermo
dc.date.available
2021-05-21T04:28:38Z
dc.date.issued
2007-06
dc.identifier.citation
Martínez Noël, Giselle María Astrid; Nagaraj, Vinay Janthakahalli; Caló, Gonzalo Federico; Wiemken, Andrés S.; Pontis, Horacio Guillermo; Sucrose regulated expression of a Ca2+-dependent protein kinase (TaCDPK1) gene in excised leaves of wheat; Elsevier France-editions Scientifiques Medicales Elsevier; Plant Physiology and Biochemistry; 45; 6-7; 6-2007; 410-419
dc.identifier.issn
0981-9428
dc.identifier.uri
http://hdl.handle.net/11336/132465
dc.description.abstract
Sucrose (Suc) can influence the expression of a large number of genes and thereby regulates many metabolic and developmental processes. However, the Suc sensing and the components of the ensuing signaling transduction pathway leading to the regulation of gene expression are not fully understood. We have shown that protein kinases and phosphatases are involved in the Suc induced expression of fructosyltransferase (FT) genes and fructan accumulation by an hexokinase independent pathway in wheat (Triticum aestivum). In the present study, using an RT-PCR based strategy, we have cloned a calcium-dependent protein kinase (TaCDPK1) cDNA that is upregulated during Suc treatment of excised wheat leaves. The deduced amino-acid sequence of CDPK1 has high sequence similarity (>70%) to known CDPKs from both monocots and dicots. Based on sequence homology, TaCDPK1 sequence shows a variable domain preceding a catalytic domain, an autoinhibitory function domain, and a C-terminal calmodulin-domain containing 4 EF-hand calcium-binding motifs, along with a N-myristoylation motif in the N-terminal variable domain. The recombinant Escherichia coli expressed TaCDPK1 was able to phosphorylate histone III-S in a calcium dependent manner in in vitro assays. The TaCDPK1 gene expression, as determined by quantitative RT-PCR, is induced by Suc and this effect is repressed by the inhibitors of the putative components of the Suc signal transduction pathway (calcium, Ser/Thr protein kinases and protein phosphatases). We propose that TaCDPK1 is involved in the Suc induced signaling pathway in wheat leaves.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier France-editions Scientifiques Medicales Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CALCIUM-DEPENDENT PROTEIN KINASE
dc.subject
FRUCTAN
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SUGAR SIGNALING
dc.subject
TRITICUM AESTIVUM
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Sucrose regulated expression of a Ca2+-dependent protein kinase (TaCDPK1) gene in excised leaves of wheat
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-04-28T21:44:04Z
dc.journal.volume
45
dc.journal.number
6-7
dc.journal.pagination
410-419
dc.journal.pais
Francia
dc.journal.ciudad
Paris
dc.description.fil
Fil: Martínez Noël, Giselle María Astrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Nagaraj, Vinay Janthakahalli. Arizona State University; Estados Unidos
dc.description.fil
Fil: Caló, Gonzalo Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Wiemken, Andrés S.. Botanisches Institut der Universität Basel; Suiza
dc.description.fil
Fil: Pontis, Horacio Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.journal.title
Plant Physiology and Biochemistry
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0981942807000502
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1016/j.plaphy.2007.03.004
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