Artículo
Antioxidant and molecular chaperone defenses during estivation and arousal in the South American apple-snail Pomacea canaliculata
Giraud Billoud, Maximiliano German
; Vega, Israel Aníbal
; Rinaldi Tosi, Martin Edgardo
; Abud, María A.; Calderón, María L.; Castro-Vazquez, Alfredo
Fecha de publicación:
01/2013
Editorial:
Company Of Biologists Ltd
Revista:
Journal Of Experimental Biology
ISSN:
0022-0949
http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=23077161
http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=23077161
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The invasive Pomacea canaliculata estivates during periods of drought and should cope with harmful effects of reoxygenation during arousal. We studied thiobarbituric acid reactive substances (TBARS), enzymatic (superoxide dismutase, SOD and catalase, CAT) and non-enzymatic ontioxidants (uric acid and reduced glutathione), and heat shock protein expression (Hsc70, Hsp70 and Hsp90) in (1) active control snails, (2) snails after 45 days of estivation, and (3) aroused snails 20 min and (4) 24 h after water exposure, in midgut gland, kidney and foot. Both kidney and foot (but not the midgut gland) showed a TBARS increase during estivation and a decrease after arousal. Tissue SOD and CAT did not change in any experimental groups. Uric acid increased during estivation in all tissues, and it decreased after arousal in the kidney. Allantoin, the oxidation product of uric acid, remained constant in the midgut gland but it decreased in the kidney until 20 min after arousal; however, allantoin levels rose in both kidney and foot 24 h after arousal. Reduced glutathione decreased during estivation and arousal, in both midgut gland and kidney, and it remained constant in the foot. Hsc70 and Hsp70 kidney levels were stable during the activity-estivation cycle and Hsp90 expression decrease during estivation and recovers in the early arousal. In foot, the expression of Hsp70 and Hsp90 was high during activity and estivation periods and disminished after arousal. Results indicate that a panoply of antioxidant and molecular chaperone defenses may be involved during the estivation-arousal cycle in this freshwater gastropod.
Palabras clave:
Oxidative Stress
,
Oxyradicals
,
Uric Acid
,
Gastropods
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - MENDOZA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MENDOZA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - MENDOZA
Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Giraud Billoud, Maximiliano German; Vega, Israel Aníbal; Rinaldi Tosi, Martin Edgardo; Abud, María A. ; Calderón, María L.; et al.; Antioxidant and molecular chaperone defenses during estivation and arousal in the South American apple-snail Pomacea canaliculata; Company Of Biologists Ltd; Journal Of Experimental Biology; 216; 1-2013; 614-622
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