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dc.contributor.author
Bouchard, Damien S.
dc.contributor.author
Seridan, Bianca
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Saraoui, Taous
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Rault, Lucie
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Germon, Pierre
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Gonzalez Moreno, Candelaria
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Nader Macias, Fátima M. E.
dc.contributor.author
Baud, Damien
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François, Patrice
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Chuat, Victoria
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Chain, Florian
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Langella, Philippe
dc.contributor.author
Nicoli, Jacques
dc.contributor.author
Le Loir, Yves
dc.contributor.author
Even, Sergine
dc.date.available
2017-02-10T17:23:03Z
dc.date.issued
2015-12
dc.identifier.citation
Bouchard, Damien S.; Seridan, Bianca; Saraoui, Taous; Rault, Lucie; Germon, Pierre; et al.; Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis; Public Library Of Science; Plos One; 10; 12; 12-2015; 1-18; e0144831
dc.identifier.issn
1932-6203
dc.identifier.uri
http://hdl.handle.net/11336/12839
dc.description.abstract
Bovine mastitis is a costly disease in dairy cattle worldwide. As of yet, the control of bovine mastitis is mostly based on prevention by thorough hygienic procedures during milking. Additional strategies include vaccination and utilization of antibiotics. Despite these measures, mastitis is not fully under control, thus prompting the need for alternative strategies. The goal of this study was to isolate autochthonous lactic acid bacteria (LAB) from bovine mammary microbiota that exhibit beneficial properties that could be used for mastitis prevention and/or treatment. Sampling of the teat canal led to the isolation of 165 isolates, among which a selection of ten non-redundant LAB strains belonging to the genera Lactobacillus and Lactococcus were further characterized with regard to several properties: surface properties (hydrophobicity, autoaggregation); inhibition potential of three main mastitis pathogens, Staphylococcus aureus, Escherichia coli and Streptococcus uberis; colonization capacities of bovine mammary epithelial cells (bMEC); and immunomodulation properties. Three strains, Lactobacillus brevis 1595 and 1597 and Lactobacillus plantarum 1610, showed high colonization capacities and a medium surface hydrophobicity. These strains are good candidates to compete with pathogens for mammary gland colonization. Moreover, nine strains exhibited anti-inflammatory properties, as illustrated by the lower IL-8 secretion by E. coli-stimulated bMEC in the presence of these LAB. Full genome sequencing of five candidate strains allowed to check for undesirable genetic elements such as antibiotic resistance genes and to identify potential bacterial determinants involved in the beneficial properties. This large screening of beneficial properties while checking for undesirable genetic markers allowed the selection of promising candidate LAB strains from bovine mammary microbiota for the prevention and/or treatment of bovine mastitis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Lactic Acid Bacteria
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Bovine
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Mastitis
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Probiotic
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Lactic acid bacteria isolated from bovine mammary microbiota: potential allies against bovine Mastitis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-02-09T17:55:34Z
dc.journal.volume
10
dc.journal.number
12
dc.journal.pagination
1-18; e0144831
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Bouchard, Damien S.. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
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Fil: Seridan, Bianca. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia. Universidade Federal do Minas Gerais; Brasil
dc.description.fil
Fil: Saraoui, Taous. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Rault, Lucie. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Germon, Pierre. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Gonzalez Moreno, Candelaria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
dc.description.fil
Fil: Nader Macias, Fátima M. E.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
dc.description.fil
Fil: Baud, Damien. Geneva University Hospital; Suiza
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Fil: François, Patrice. Geneva University Hospital; Suiza
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Fil: Chuat, Victoria. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Chain, Florian. INRA-MICALIS; Francia
dc.description.fil
Fil: Langella, Philippe. INRA-MICALIS; Francia
dc.description.fil
Fil: Nicoli, Jacques. Universidade Federal do Minas Gerais; Brasil
dc.description.fil
Fil: Le Loir, Yves. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
dc.description.fil
Fil: Even, Sergine. Agrocampus Ouest; Francia. Institut National de la Recherche Agronomique; Francia
dc.journal.title
Plos One
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pone.0144831
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0144831
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694705/
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