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dc.contributor.author
Waks Serra, María Victoria
dc.contributor.author
Grosenick, Dirk
dc.contributor.author
Macdonald, Rainer
dc.contributor.author
Pomarico, Juan Antonio
dc.contributor.author
Iriarte, Daniela Ines
dc.date.available
2020-08-11T17:47:56Z
dc.date.issued
2019-06-25
dc.identifier.citation
Waks Serra, María Victoria; Grosenick, Dirk; Macdonald, Rainer; Pomarico, Juan Antonio; Iriarte, Daniela Ines; A systematic study on fluorescence contrast in near infrared diffuse transmittance imaging with indocyanine green; N I R Publications; Journal of Near Infrared Spectroscopy; 27; 5; 25-6-2019; 333-344
dc.identifier.issn
0967-0335
dc.identifier.uri
http://hdl.handle.net/11336/111424
dc.description.abstract
Near infrared fluorescence imaging is a sensitive, noninvasive technique for diagnostic applications in biomedical optics. The main purpose of this work is thus to explore how to improve the contrast of images obtained by near infrared light using a fluorescent extrinsic agent. Among different fluorophores, indocyanine green has been mostly studied because it is approved for use in humans. In this work, simulations and experimental studies on phantoms (systems that optically emulate biological tissues) are used to systematically investigate the influence of the increased intrinsic tissue absorption by adding indocyanine green. The experiments reproduce the situation of fluorescence imaging of carcinomas in the human breast, where the natural absorption due to neovascularization is increased by the injection of this fluorophore. Assuming measurements in transmission geometry, the breast is modeled by a homogeneous background medium containing a tumor-like inclusion (or lesion) with two- or threefold increased absorption. Fluorescence contrast is simulated over a broad range of dye concentrations using diffusion theory. Selected concentrations ratios are applied in experimental studies with laser excitation of indocyanine green fluorescence and with a charge-coupled device camera for fluorescence detection. Both simulations and experiments show that the intrinsic absorption of the inclusion strongly reduces the number of detected fluorescence photons and that the fluorescence contrast can be canceled or become even negative. It was found that for typical optical properties and geometrical conditions, in fluorescence imaging of breast cancer, a dye ratio of about 10:1 (lesion:background) is required to turn from negative to positive fluorescence contrast. Since such a high ratio is difficult to attain, raw fluorescence images need to be normalized by the intrinsic lesion absorption (without indocyanine green (ICG)) to enhance the presence of the dye in the lesion.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
N I R Publications
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BIOMEDICAL OPTICS
dc.subject
FLUORESCENCE
dc.subject
BREAST TUMOR
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ICG
dc.subject
TURBID MEDIA
dc.subject.classification
Óptica
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Ciencias Físicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
A systematic study on fluorescence contrast in near infrared diffuse transmittance imaging with indocyanine green
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-23T19:24:36Z
dc.identifier.eissn
1751-6552
dc.journal.volume
27
dc.journal.number
5
dc.journal.pagination
333-344
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Waks Serra, María Victoria. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina
dc.description.fil
Fil: Grosenick, Dirk. Physikalisch Technische Bundesanstalt; Alemania
dc.description.fil
Fil: Macdonald, Rainer. Physikalisch Technische Bundesanstalt; Alemania
dc.description.fil
Fil: Pomarico, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina
dc.description.fil
Fil: Iriarte, Daniela Ines. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina
dc.journal.title
Journal of Near Infrared Spectroscopy
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.1177/0967033519857733
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1177/0967033519857733
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