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dc.contributor.author
Lederer, Sabine
dc.contributor.author
Lattwein, Erik
dc.contributor.author
Hanke, Merle
dc.contributor.author
Sonnenberg, Karen
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Stoecker, Winfried
dc.contributor.author
Lundkvist, Åke
dc.contributor.author
Vaheri, Antti
dc.contributor.author
Vapalahti, Olli
dc.contributor.author
Chan, Paul K. S.
dc.contributor.author
Feldmann, Heinz
dc.contributor.author
Dick, Daryl
dc.contributor.author
Schmidt Chanasit, Jonas
dc.contributor.author
Padula, Paula Julieta
dc.contributor.author
Vial, Pablo A.
dc.contributor.author
Panculescu Gatej, Raluca
dc.contributor.author
Ceianu, Cornelia
dc.contributor.author
Heyman, Paul
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Avšič Županc, Tatjana
dc.contributor.author
Niedrig, Matthias
dc.date.available
2017-01-10T14:34:37Z
dc.date.issued
2013-04
dc.identifier.citation
Lederer, Sabine; Lattwein, Erik; Hanke, Merle; Sonnenberg, Karen; Stoecker, Winfried; et al.; Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses; Public Library Of Science; Neglected Tropical Diseases; 7; 4-2013; 1-9
dc.identifier.issn
1935-2735
dc.identifier.uri
http://hdl.handle.net/11336/11026
dc.description.abstract
In order to detect serum antibodies against clinically important Old and New World hantaviruses simultaneously, multiparametric indirect immunofluorescence assays (IFAs) based on biochip mosaics were developed. Each of the mosaic substrates consisted of cells infected with one of the virus types Hantaan (HTNV), Puumala (PUUV), Seoul (SEOV), Saaremaa (SAAV), Dobrava (DOBV), Sin Nombre (SNV) or Andes (ANDV). For assay evaluation, serum IgG and IgM antibodies were analyzed using 184 laboratory-confirmed hantavirus-positive sera collected at six diagnostic centers from patients actively or previously infected with the following hantavirus serotypes: PUUV (Finland, n = 97); SEOV (China, n = 5); DOBV (Romania, n = 7); SNV (Canada, n = 23); ANDV (Argentina and Chile, n = 52). The control panel comprised 89 sera from healthy blood donors. According to the reference tests, all 184 patient samples were seropositive for hantavirus-specific IgG (n = 177; 96%) and/or IgM (n = 131; 72%), while all control samples were tested negative. In the multiparametric IFA applied in this study, 183 (99%) of the patient sera were IgG and 131 (71%) IgM positive (accordance with the reference tests: IgG, 96%; IgM, 93%). Overall IFA sensitivity for combined IgG and IgM analysis amounted to 100% for all serotypes, except for SNV (96%). Of the 89 control sera, 2 (2%) showed IgG reactivity against the HTNV substrate, but not against any other hantavirus. Due to the high cross-reactivity of hantaviral nucleocapsid proteins, endpoint titrations were conducted, allowing serotype determination in .90% of PUUV- and ANDV-infected patients. Thus, multiparametric IFA enables highly sensitive and specific serological diagnosis of hantavirus infections and can be used to differentiate PUUV and ANDV infection from infections with Murinae-borne hantaviruses (e.g. DOBV and SEOV).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Public Library Of Science
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Monoclonals
dc.subject
Hantavirus
dc.subject
Elisa
dc.subject.classification
Virología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Indirect Immunofluorescence Assay for the Simultaneous Detection of Antibodies against Clinically Important Old and New World Hantaviruses
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-24T18:38:30Z
dc.journal.volume
7
dc.journal.pagination
1-9
dc.journal.pais
Estados Unidos
dc.journal.ciudad
San Francisco
dc.description.fil
Fil: Lederer, Sabine. EUROIMMUN Medizinische Labordiagnostika; Alemania
dc.description.fil
Fil: Lattwein, Erik. EUROIMMUN Medizinische Labordiagnostika; Alemania
dc.description.fil
Fil: Hanke, Merle. EUROIMMUN Medizinische Labordiagnostika; Alemania
dc.description.fil
Fil: Sonnenberg, Karen. EUROIMMUN Medizinische Labordiagnostika; Alemania
dc.description.fil
Fil: Stoecker, Winfried. EUROIMMUN Medizinische Labordiagnostika; Alemania
dc.description.fil
Fil: Lundkvist, Åke. Karolinska Huddinge Hospital. Karolinska Institutet; Suecia
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Fil: Vaheri, Antti. University of Helsinki; Finlandia
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Fil: Vapalahti, Olli. University of Helsinki; Finlandia
dc.description.fil
Fil: Chan, Paul K. S.. Chinese University of Hong Kong; República de China
dc.description.fil
Fil: Feldmann, Heinz. Canadian Science Centre for Human and Animal Health. Public Health Agency of Canada; Canadá
dc.description.fil
Fil: Dick, Daryl. Canadian Science Centre for Human and Animal Health. Public Health Agency of Canada; Canadá
dc.description.fil
Fil: Schmidt Chanasit, Jonas. Bernhard Nocht Institute for Tropical Medicine; Alemania
dc.description.fil
Fil: Padula, Paula Julieta. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Vial, Pablo A.. Universidad del Desarrollo; Chile
dc.description.fil
Fil: Panculescu Gatej, Raluca. CANTACUZINO National Institute for Research and Development in Microbiology and Immunology; Rumania
dc.description.fil
Fil: Ceianu, Cornelia. CANTACUZINO National Institute for Research and Development in Microbiology and Immunology; Rumania
dc.description.fil
Fil: Heyman, Paul. Queen Astrid Military Hospital; Bélgica
dc.description.fil
Fil: Avšič Županc, Tatjana. Institute of Microbiology and Immunology; Eslovenia
dc.description.fil
Fil: Niedrig, Matthias. Robert Koch Institute; Alemania
dc.journal.title
Neglected Tropical Diseases
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0002157
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593524/
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1371/journal.pntd.0002157
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