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dc.contributor.author
Costa, Hernán
dc.contributor.author
Rodríguez Gastón, Jorgelina Andrea
dc.contributor.author
Lara, María Julia
dc.contributor.author
Martinez Ortiz, Camila
dc.contributor.author
Moriwaki, Cristiane
dc.contributor.author
Matioli, Graciette
dc.contributor.author
Ferrarotti, Susana Alicia
dc.date.available
2020-07-14T14:24:16Z
dc.date.issued
2015-01
dc.identifier.citation
Costa, Hernán; Rodríguez Gastón, Jorgelina Andrea; Lara, María Julia; Martinez Ortiz, Camila; Moriwaki, Cristiane; et al.; Cyclodextrin glycosyltransferase production by free cells of Bacillus circulans DF 9R in batch fermentation and by immobilized cells in a semi-continuous process.; Springer; Bioprocess And Biosystems Engineering; 1-2015; 1-11
dc.identifier.issn
1615-7591
dc.identifier.uri
http://hdl.handle.net/11336/109225
dc.description.abstract
Cyclodextrin glycosyltransferase (CGTase) catalyzes the starch conversion into cyclic or linear oligosaccharides, important industrial products. In this work, conditions to increase the enzyme production from Bacillus circulans strain DF 9R were optimized by two systems. On one hand, free cells were grown in batch fermentation experiments conducted to optimize aeration and pH. The increased production of the enzyme was achieved with aeration of 1.5 vvm at pH regulated to 7.6 after 48 h of growth. On the other hand, bacterial cells were immobilized on vegetable and synthetic sponge and used for CGTase production in a semi-continuous process, by transferring the immobilized cells to a suitable culture medium in successive cycles. An initial biomass of 30 mg of lyophilized cells and an immobilization time of 24 h with vegetal or synthetic sponge was enough to reach the greater production of the CGTase. Sponge immobilized bacteria were re-used in 12 successive cycles, although enzyme production dropped significantly after the sixth cycle reaching around 40 % of initial enzyme activity. Even though the specific productivity was similar in both processes, the semi-continuous process has advantages compared to batch process with free cells because employed an inexpensive and biodegradable matrix, thus making the system potentially appropriate for CGTase production on an industrial scale.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CYCLODEXTRIN GLYCOSYLTRANSFERASE
dc.subject
FERMENTATION
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IMMOBILIZED CELLS
dc.subject
VEGETABLE SPONGE
dc.subject.classification
Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.
dc.subject.classification
Biotecnología Industrial
dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
dc.title
Cyclodextrin glycosyltransferase production by free cells of Bacillus circulans DF 9R in batch fermentation and by immobilized cells in a semi-continuous process.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-07-13T20:54:17Z
dc.journal.pagination
1-11
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Costa, Hernán. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
dc.description.fil
Fil: Rodríguez Gastón, Jorgelina Andrea. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
dc.description.fil
Fil: Lara, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
dc.description.fil
Fil: Martinez Ortiz, Camila. Universidad Estadual de Maringá; Brasil
dc.description.fil
Fil: Moriwaki, Cristiane. Universidad Estadual de Maringá; Brasil
dc.description.fil
Fil: Matioli, Graciette. Universidad Estadual de Maringá; Brasil
dc.description.fil
Fil: Ferrarotti, Susana Alicia. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Area de Química Biológica; Argentina
dc.journal.title
Bioprocess And Biosystems Engineering
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs00449-014-1347-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00449-014-1347-6
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