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dc.contributor.author
Moreno Torrejon, Laura
dc.contributor.author
Ceballos, Laura
dc.contributor.author
Fairweather, Ian
dc.contributor.author
Lanusse, Carlos Edmundo
dc.contributor.author
Alvarez, Luis Ignacio
dc.date.available
2017-01-04T15:04:56Z
dc.date.issued
2014-02
dc.identifier.citation
Moreno Torrejon, Laura; Ceballos, Laura; Fairweather, Ian; Lanusse, Carlos Edmundo; Alvarez, Luis Ignacio; Time-course and accumulation of triclabendazole and its metabolites in bile, liver tissues and flukes collected from treated sheep; Elsevier; Experimental Parasitology; 136; 2-2014; 14-19
dc.identifier.issn
0014-4894
dc.identifier.uri
http://hdl.handle.net/11336/10797
dc.description.abstract
The flukicidal compound triclabendazole (TCBZ) has a complex metabolic pattern that includes the systemic presence of its sulphoxide (TCBZ.SO) and sulphone (TCBZ.SO2) metabolites, usually recovered from the bile of treated animals. The aim of the current work was to evaluate the time-course and pattern of in vivo accumulation of TCBZ/metabolites into adult Fasciola hepatica specimens recovered from infected sheep. Twelve (12) healthy Corriedale sheep were orally infected with one hundred (100) metacercariae of the TCBZ-susceptible Cullomptom isolate of F. hepatica. Sixteen weeks after infection, animals were intraruminally treated with TCBZ (10 mg/kg). At 3, 24, 48 and 60 h post-treatment (pt), animals were sacrificed (n = 3/time period) and samples of blood, bile, liver tissue and adult F. hepatica specimens were collected. The concentrations of TCBZ/metabolites were measured by HPLC. TCBZ.SO and TCBZ.SO2 were the only molecules recovered in the bloodstream, with peak plasma concentrations of 10.8 lg/mL (TCBZ.SO) and 12.6 lg/mL (TCBZ.SO2). The same metabolites were also the main analytes accumulated within the adult flukes, reaching peak concentrations between 6.35 lg/g (TCBZ.SO) and 13.9 lg/g (TCBZ.SO2) at 24 h pt, which was coincident with the time when the maximum plasma concentration was attained. Low levels of TCBZ parent drug (0.14 lg/g at 24 h pt) were measured within collected flukes. TCBZ parent drug and its sulpho- and hydroxy-derivatives were recovered in bile collected from treated sheep between 3 and 60 h pt. Although relatively high concentrations of hydroxy-TCBZ (ranging from 0.86 to 10.1 lg/mL) were measured in bile, this metabolite was not recovered within the flukes at any time pt. Finally, TCBZ parent drug was the main compound accumulated in liver tissue over the 60 h pt period. The time-course and drug concentration patterns within the adult liver fluke after TCBZ treatment followed a similar trend to those observed in plasma. Overall, the data reported here confirm that oral ingestion is a main route of drug entry into the trematode in vivo exposed to TCBZ/metabolites.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Triclabendazole
dc.subject
Fasciola Hepatica
dc.subject
Drug Distribution
dc.subject.classification
Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Time-course and accumulation of triclabendazole and its metabolites in bile, liver tissues and flukes collected from treated sheep
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-23T19:57:37Z
dc.journal.volume
136
dc.journal.pagination
14-19
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Moreno Torrejon, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
dc.description.fil
Fil: Ceballos, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
dc.description.fil
Fil: Fairweather, Ian. The Queens University Of Belfast; Irlanda
dc.description.fil
Fil: Lanusse, Carlos Edmundo. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Alvarez, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires; Argentina
dc.journal.title
Experimental Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.exppara.2013.10.014
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0014489413002865
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