Mostrar el registro sencillo del ítem
dc.contributor.author
Soto, Lorena Paola
dc.contributor.author
Frizzo, Laureano Sebastian
dc.contributor.author
Bertozzi, Ezequiel
dc.contributor.author
Avataneo, Elizabeth
dc.contributor.author
Sequeira, Gabriel Jorge
dc.contributor.author
Rosmini, Marcelo Raul
dc.date.available
2020-04-29T15:03:08Z
dc.date.issued
2009-09
dc.identifier.citation
Soto, Lorena Paola; Frizzo, Laureano Sebastian; Bertozzi, Ezequiel; Avataneo, Elizabeth; Sequeira, Gabriel Jorge; et al.; Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use; Hindawi Publishing Corporation; Veterinary Medicine International; 2010; 9-2009; 1-7
dc.identifier.issn
2042-0048
dc.identifier.uri
http://hdl.handle.net/11336/103898
dc.description.abstract
The intestinal microbiota has an influence on the growth and health status of the hosts. This is of particular interest in animals reared using intensive farming practices. Hence, it is necessary to know more about complexity of the beneficial intestinal microbiota. The use of molecular methods has revolutionized microbial identification by improving its quality and effectiveness. The specific aim of the study was to analyze predominant species of Lactobacillus in intestinal microbial ecosystem of young calves. Forty-two lactic acid bacteria (LAB) isolated from intestinal tract of young calves were characterized by: Amplified Ribosomal DNA Restriction Analysis (ARDRA), by using Hae III, Msp I, and Hinf I restriction enzymes, and 16S rDNA gene sequencing. ARDRA screening revealed nine unique patterns among 42 isolates, with the same pattern for 29 of the isolates. Gene fragments of 16S rDNA of 19 strains representing different patterns were sequenced to confirm the identification of these species. These results confirmed that ARDRA is a good tool for identification and discrimination of bacterial species isolated from complex ecosystem and between closely related groups. This paper provides information about the LAB species predominant in intestinal tract of young calves that could provide beneficial effects when administered as probiotic
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Hindawi Publishing Corporation
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Molecular microbial analysis
dc.subject
Lactobacillus
dc.subject
Calves
dc.subject
Probiotic
dc.subject.classification
Biotecnología Agrícola y Biotecnología Alimentaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Molecular Microbial Analysis of Lactobacillus Strains Isolated from the Gut of Calves for Potential Probiotic Use
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2020-04-28T13:07:35Z
dc.journal.volume
2010
dc.journal.pagination
1-7
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
dc.description.fil
Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
dc.description.fil
Fil: Bertozzi, Ezequiel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
dc.description.fil
Fil: Avataneo, Elizabeth. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
dc.description.fil
Fil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
dc.description.fil
Fil: Rosmini, Marcelo Raul. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina
dc.journal.title
Veterinary Medicine International
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sage-hindawi.com/journals/vmi/2010/274987.html
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4061/2010/274987
Archivos asociados