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dc.contributor.author
Gomes, Bárbara
dc.contributor.author
Gonçalves, Sónia
dc.contributor.author
Disalvo, Edgardo Anibal
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dc.contributor.author
Hollmann, Axel
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dc.contributor.author
Santos, Nuno C.
dc.date.available
2020-01-09T20:46:17Z
dc.date.issued
2018-05
dc.identifier.citation
Gomes, Bárbara; Gonçalves, Sónia; Disalvo, Edgardo Anibal; Hollmann, Axel; Santos, Nuno C.; Effect of 25-hydroxycholesterol in viral membrane fusion: Insights on HIV inhibition; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1860; 5; 5-2018; 1171-1178
dc.identifier.issn
0005-2736
dc.identifier.uri
http://hdl.handle.net/11336/94246
dc.description.abstract
Recently, it was demonstrated that 25-hydroxycholesterol (25HC), an oxidized cholesterol derivative, inhibits human immunodeficiency virus type 1 (HIV) entry into its target cells. However, the mechanisms involved in this action have not yet been established. The aim of this work was to study the effects of 25HC in biomembrane model systems and at the level of HIV fusion peptide (HIV-FP). Integration of different biophysical approaches was made in the context of HIV fusion process, to clarify the changes at membrane level due to the presence of 25HC that result in the suppressing of viral infection. Lipid vesicles mimicking mammalian and HIV membranes were used on spectroscopy assays and lipid monolayers in surface pressure studies. Peptide-induced lipid mixing assays were performed by Förster resonance energy transfer to calculate fusion efficiency. Liposome fusion is reduced by 50% in the presence of 25HC, comparatively to cholesterol. HIV-FP conformation was assessed by infrared assays and it relies on sterol nature. Anisotropy, surface pressure and dipole potential assays indicate that the conversion of cholesterol in 25HC leads to a loss of the cholesterol modulating effect on the membrane. With different biophysical techniques, we show that 25HC affects the membrane fusion process through the modification of lipid membrane properties, and by direct alterations on HIV-FP structure. The present data support a broad antiviral activity for 25HC.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
25-HYDROXYCHOLESTEROL
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CHOLESTEROL
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FLUORESCENCE SPECTROSCOPY
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HUMAN IMMUNODEFICIENCY VIRUS
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INFRARED SPECTROSCOPY
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PRESSURE STUDIES
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Biofísica
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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dc.title
Effect of 25-hydroxycholesterol in viral membrane fusion: Insights on HIV inhibition
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-28T18:40:41Z
dc.journal.volume
1860
dc.journal.number
5
dc.journal.pagination
1171-1178
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gomes, Bárbara. Universidade de Lisboa; Portugal
dc.description.fil
Fil: Gonçalves, Sónia. Universidade de Lisboa; Portugal
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Fil: Disalvo, Edgardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
dc.description.fil
Fil: Hollmann, Axel. Universidade de Lisboa; Portugal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Microbiología Molecular; Argentina
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Fil: Santos, Nuno C.. Universidade de Lisboa; Portugal
dc.journal.title
Biochimica et Biophysica Acta - Biomembranes
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0005273618300427
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.bbamem.2018.02.001
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