Disease incidence of charcoal rot (Macrophomina phaseolina) on soybean in north-western Argentina and genetic characteristics of the pathogen

Abstract

Charcoal rot of soybean (Glycine max (L.) Merr.), caused by Macrophomina phaseolina (Tassi) Goid., is an economically important disease worldwide. In recent years, charcoal rot has become a concern for farmers in north-western Argentina. The present work aimed to (1) evaluate disease incidence of charcoal rot; (2) assess morphological and genetic characteristics of M. phaseolina isolates from soybean and other hosts collected in north-western Argentina; and (3) compare genetic diversity between soybean isolates of M. phaseolina from Argentina and the USA. Incidence of charcoal rot evaluated in 11 locations during five growing seasons (2008–2012) was 1%, 1%, 1%, 5–10% and 90%, respectively, indicating a gradual increase over the cropping seasons. Cluster analysis by AFLP of Argentinean isolates exhibited one main group, with isolate Mp56 separated at a genetic distance of 0.70. Four main groups were identified using SSR markers at the same genetic distance, with Mp56 and Mp53 separated from them. There was no clear association between AFLP, SSR profiles, morphological characteristics or host of origin within isolates from Argentina. However, using principal coordinate analysis of SSRs, 22 isolates of M. phaseolina from soybean from north-western Argentina were clearly distinguished from 11 isolates previously collected from two states in the USA. Our results confirmed the increasing importance of charcoal rot in north-western Argentina and identified characteristics of isolates that may be useful for breeding for disease resistance and developing integrated management programmes for charcoal rot in Argentina in the future.