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dc.contributor.author
Wagenknecht, Martin
dc.contributor.author
Dib, Julian Rafael
dc.contributor.author
Thurmer, Andrea
dc.contributor.author
Rolf, Daniel
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Farias, Maria Eugenia
dc.contributor.author
Meinhardt, Friedhelm
dc.date.available
2019-12-12T19:19:51Z
dc.date.issued
2010-12
dc.identifier.citation
Wagenknecht, Martin; Dib, Julian Rafael; Thurmer, Andrea ; Rolf, Daniel; Farias, Maria Eugenia; et al.; Structural peculiarities of linear megaplasmid, pLMA1, from Micrococcus luteus interfere with pyrosequencing reads assembly; Springer; Biotechnology Letters; 32; 12; 12-2010; 1853-1862
dc.identifier.issn
0141-5492
dc.identifier.uri
http://hdl.handle.net/11336/92108
dc.description.abstract
Different strains of Micrococcus luteus, isolated from high-altitude Argentinean wetlands, were recently reported to harbour the linear plasmids pLMA1, pLMH5 and pLMV7, all of which with 5′-covalently attached terminal proteins. The link between pLMA1 and the host's erythromycin resistance as well as further presumptive qualities prompted us to perform a detailed characterization. When the 454 technology was applied for direct sequencing of gel-purified pLMA1, assembly of the reads was impossible. However, combined Sanger/454 sequencing of cloned pLMA1 fragments, covering altogether 23 kb of the 110-kb spanning plasmid, allowed numerous sequence repeats of varying in lengths to be identified thus rendering an explanation for the above 454 assembly failure. A large number of putative transposase genes were identified as well. Furthermore, a region with five putative iteron sequences is possibly involved in pLMA1 replication. © 2010 The Author(s).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
454 SEQUENCING
dc.subject
INVERTED REPEATS
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ITERON
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LINEAR PLASMID
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MICROCOCCUS REPETITIVE SEQUENCES
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TRANSPOSASE
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Structural peculiarities of linear megaplasmid, pLMA1, from Micrococcus luteus interfere with pyrosequencing reads assembly
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-12-11T18:47:48Z
dc.journal.volume
32
dc.journal.number
12
dc.journal.pagination
1853-1862
dc.journal.pais
Alemania
dc.journal.ciudad
Holanda
dc.description.fil
Fil: Wagenknecht, Martin. Westfalische Wilhelms Universitat; Alemania
dc.description.fil
Fil: Dib, Julian Rafael. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Thurmer, Andrea. Universität Göttingen; Alemania
dc.description.fil
Fil: Rolf, Daniel. Universität Göttingen; Alemania
dc.description.fil
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Meinhardt, Friedhelm. Westfalische Wilhelms Universitat; Alemania
dc.journal.title
Biotechnology Letters
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://dx.doi.org/10.1007/s10529-010-0357-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs10529-010-0357-y


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