The Effect of Seasonal Humidity on Survival and Duration of Dormancy on Diverging Mexican Rhagoletis pomonella (Diptera: Tephritidae) Populations Inhabiting Different Environments

Abstract

Phytophagous insects synchronize emergence with plant phenology by engaging in dormancy during periods of host scarcity and environmental stress. Regulation of dormancy is achieved through response to seasonal cues. While temperature and photoperiod are important cues in temperate latitudes, seasonal humidity, such as the onset of rains, can be a reliable cue to for synchronization of emergence and affects survival of overwintering insects. We compared response of Mexican Rhagoletis pomonella (Walsh) (Diptera: Tephritidae) populations inhabiting subtropical environments differing in humidity patterns, to seasonal humidity regimes. Both populations emerged as adults in high proportions and suffered lower mortality under humidity regimes ending with a humid summer, but the effect was more pronounced for the Eje Volcanico Trans Mexicano (EVTM) population, which inhabits a dryer environment and undergoes longer dormancy. While there were no differences among pupae from the Sierra Madre Oriental (SMO) in percent of non-emerged pupae surviving and engaging in prolonged dormancy after a year, EVTM pupae exposed to an initial humid period engaged in prolonged dormancy in higher proportions than those exposed to other regimes. Seasonal humidity had little effect on the duration of dormancy, but EVTM pupae exposed to consecutive dry periods took longer to emerge than those exposed to other regimes. Our results suggest that rather than being used as a token stimulus, humidity affected survival of overwintering R. pomonella, especially at the end of dormancy when energy reserves are depleted and there is an increase in metabolic rate that renders EVTM pupae more susceptible to desiccation.