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dc.contributor.author
Regner, Erika Lucía
dc.contributor.author
Thompson, Carolina S.
dc.contributor.author
Iglesias, Alberto Alvaro
dc.contributor.author
Guerrero, Sergio Adrian
dc.contributor.author
Arias, Diego Gustavo
dc.date.available
2016-12-07T17:22:23Z
dc.date.issued
2014-04
dc.identifier.citation
Regner, Erika Lucía; Thompson, Carolina S.; Iglesias, Alberto Alvaro; Guerrero, Sergio Adrian; Arias, Diego Gustavo; Biochemical Characterization of the Thioredoxin Reductase from Babesia bovis; Elsevier Masson; Biochimie; 99; 4-2014; 44-53
dc.identifier.issn
0300-9084
dc.identifier.uri
http://hdl.handle.net/11336/8994
dc.description.abstract
This paper addresses the identification, cloning, expression, purification and functional characterization of thioredoxin reductase from Babesia bovis, the etiological agent of babesiosis. The work deals with in vitro steady state kinetic studies and other complementary analyses of the thioredoxin reductase found in the pathogenic protist. Thioredoxin reductase from B. bovis was characterized as a homodimeric flavoprotein that catalyzes the NADPH-dependent reduction of Trx with a high catalytic efficiency. Moreover, the enzyme exhibited a disulfide reductase activity using DTNB as substrate, being this activity highly sensitive to inhibition by Eosin B. The thioredoxin reductase/thioredoxin system can reduce oxidized glutathione and S-nitrosoglutathione. Our in vitro data suggest that antioxidant defense in B. bovis could be supported by this enzyme.We have performed an enzymatic characterization, searching for targets for rational design of inhibitors. This work contributes to the better understanding of the redox biochemistry occurring in the parasite.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Masson
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Babesia
dc.subject
Eosin B
dc.subject
Nitrosoglutathione
dc.subject
Thioredoxin System
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Glutathione Disulfide
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Biochemical Characterization of the Thioredoxin Reductase from Babesia bovis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-11-23T20:14:34Z
dc.journal.volume
99
dc.journal.pagination
44-53
dc.journal.pais
Francia
dc.journal.ciudad
Paris
dc.description.fil
Fil: Regner, Erika Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
dc.description.fil
Fil: Thompson, Carolina S.. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Santa Fe. Estacion Experimental Agropecuaria Rafaela; Argentina
dc.description.fil
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
dc.description.fil
Fil: Guerrero, Sergio Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
dc.description.fil
Fil: Arias, Diego Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe. Instituto de Agrobiotecnologia del Litoral; Argentina
dc.journal.title
Biochimie
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0300908413003982#
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.biochi.2013.11.002


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