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dc.contributor.author
Zanini, Andrea Alejandra
dc.contributor.author
Cuellar, Wilmer
dc.contributor.author
Celli, Marcos Giovani
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Luque, Andres Vicente
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Medina, Ricardo Daniel
dc.contributor.author
Conci, Vilma Cecilia
dc.contributor.author
Di Feo, Liliana del Valle
dc.date.available
2019-11-21T12:18:50Z
dc.date.issued
2018-10-17
dc.identifier.citation
Zanini, Andrea Alejandra; Cuellar, Wilmer; Celli, Marcos Giovani; Luque, Andres Vicente; Medina, Ricardo Daniel; et al.; Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina; Wiley Blackwell Publishing, Inc; Plant Pathology; 67; 8; 17-10-2018; 1814-1820
dc.identifier.issn
0032-0862
dc.identifier.uri
http://hdl.handle.net/11336/89361
dc.description.abstract
Cassava common mosaic disease (CCMD) has been reported in all regions where cassava is grown in the Americas and the causal agent, Cassava common mosaic virus (CsCMV), has been identified as a mechanically transmitted potexvirus (Alphaflexiviridae). In Argentina, cassava is grown mainly in the northeast (NEA) region that shares borders with Brazil and Paraguay. Increasing incidences of CCMD were observed during the years 2014 to 2016 associated with severe leaf mosaic symptoms and yield reductions where the occurrence of CsCMV was confirmed by RT-PCR and sequencing. In this work, the virus has been successfully purified and a double-antibody sandwich (DAS-) ELISA test has been developed from an Argentinean isolate of CsCMV to extend the diagnostics of the disease. A collection of 726 samples was screened and CsCMV was detected with 100% prevalence in the NEA region. Additional co-infecting viruses were detected in some plants (64.4%); in these, CCMD symptoms correlated with CsCMV only, although more severe symptoms could be observed in mixed infected plants. Sequence analysis of the conserved RdRp domain showed a wider diversity of CsCMV isolates. Interestingly, a separate phylogenetic cluster was formed by isolates from the NEA region that only shared 77.1% to 80.3% nucleotide identity with the other clusters. These results indicate the presence of mixed strains occurring in the NEA region and suggest the presence of geographically distinct strains of CsCMV in South America.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley Blackwell Publishing, Inc
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
CASSAVA COMMON MOSAIC DISEASE
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MANIHOT ESCULENTA
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RE-EMERGENT VIRUS
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VIRUS SURVEILLANCE
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Otras Agricultura, Silvicultura y Pesca
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Agricultura, Silvicultura y Pesca
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CIENCIAS AGRÍCOLAS
dc.title
Distinct strains of the re-emergent Cassava common mosaic virus (genus: Potexvirus) infecting cassava in Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2019-10-22T17:32:05Z
dc.identifier.eissn
1365-3059
dc.journal.volume
67
dc.journal.number
8
dc.journal.pagination
1814-1820
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Zanini, Andrea Alejandra. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
dc.description.fil
Fil: Cuellar, Wilmer. International Center for Tropical Agriculture; Colombia
dc.description.fil
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Luque, Andres Vicente. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
dc.description.fil
Fil: Medina, Ricardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Conci, Vilma Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.description.fil
Fil: Di Feo, Liliana del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
dc.journal.title
Plant Pathology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12869
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12869
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1111/ppa.12869