Artículo
Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid
Fecha de publicación:
02/2019
Editorial:
Academic Press Inc Elsevier Science
Revista:
Ecotoxicology and Environmental Safety
ISSN:
0147-6513
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Due to their antimicrobial properties Copper nanoparticles (CuNps) have been proposed to be used in agriculture for pest control. Pesticides removal is mainly done by microorganisms, whose genes usually are found in degradative plasmids transferable by conjugation. Our objective was to evaluate if subinhibitory concentrations of CuNps affect the conjugation frequency (CF) of two degradative plasmids, pJP4 and pADP1. CuNps were characterized by scanning electron microscopy with X-ray detector, dynamic light scattering and atomic absorption spectrophotometry. Mating pairs were incubated in liquid media supplemented with subinhibitory concentrations of CuNps (nominal size 40-60 nm) or copper sulphate (CuSO4) as salt form. Transconjugants strains were checked for pesticide degradation and presence of plasmidial genes by PCR. CuNps dissolution in LB broth were quickly (less 3 h), meanwhile in saline medium without organic matter dissolution never pass over 20%. Neither nanoparticles nor copper salt modify the bacterial growth under subinhibitory concentrations assessed. However, under low concentrations of both copper forms (20 µg mL-1), the conjugation frequencies of degradative plasmids were reduced to 90 %. Therefore, our results indicate that ionic copper affect transference of catabolic plasmids as a subinhibitory consequence. The intentional or accidental release of copper to the environment would be affecting the biodegradative potential of microbial communities and inevitably producing as a result a decrease in the pesticides removal.
Palabras clave:
Copper
,
Nanoparticules
,
Conjugation
,
Plasmid
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(PROIMI)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Articulos de PLANTA PILOTO DE PROC.IND.MICROBIOLOGICOS (I)
Citación
Parra, Boris; Tortella, Gonzalo R.; Cuozzo, Sergio Antonio; Martínez, Miguel; Negative effect of copper nanoparticles on the conjugation frequency of degradative catabolic plasmid; Academic Press Inc Elsevier Science; Ecotoxicology and Environmental Safety; 169; 3; 2-2019; 662-668
Compartir
Altmétricas